Document Detail


Increased proteolysis in chromatin of terminally differentiated and quiescent cells.
MedLine Citation:
PMID:  6370706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endogenous proteolysis in chromatin of terminally differentiated, quiescent, and actively proliferating cells was studied by measuring the released acid-soluble radioactivity of [3H]tryptophan-prelabelled nuclear proteins, and by following the specific quantitative and qualitative changes in electrophoregrams of chromosomal proteins. The experiments suggest that the chromatin of differentiated mouse kidney and liver cells, as well as chromatin from Friend cells induced to commit terminal differentiation, exhibit increased proteolysis in comparison with that of chromatin isolated from actively proliferating cells. Enhanced proteolysis was found also for the slowly renewing and quiescent cells from adult mice. The control experiments designated to discriminate between the two possible alternatives explaining the difference--increased activity of the proteolytic enzymes associated with chromatin, or increased susceptibility of the chromosomal proteins to proteases--supported the latter alternative.
Authors:
L P Djondjurov; N Y Yancheva; E C Ivanova; K Christov
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  152     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-06-05     Completed Date:  1984-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  134-47     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells
Cell Differentiation*
Cell Division*
Chromatin / metabolism*
Chromosomal Proteins, Non-Histone / metabolism
Histones / metabolism
Interphase*
Kidney / cytology
Leukemia, Erythroblastic, Acute
Liver / cytology
Mice
Mice, Inbred C57BL
Peptide Hydrolases / metabolism
Spleen / cytology
Chemical
Reg. No./Substance:
0/Chromatin; 0/Chromosomal Proteins, Non-Histone; 0/Histones; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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