Document Detail


Increased protein yields from Escherichia coli using pressure-cycling technology.
MedLine Citation:
PMID:  16741245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sample preparation is critical to the success of two-dimensional gel electrophoresis and other analytical methods. Pressure-cycling technology (PCT) uses alternating cycles of high and low pressure to induce cell lysis. Cell suspensions were placed in PULSE Tubes and subjected to alternating cycles of high and low pressure in a Barocycler instrument. each cycle consisted of 20 sec at 35,000 psi followed by 20 sec at ambient pressure. For the bacterium Escherichia coli, PCT extracted 14.2% more total protein than was extracted using a standard bead mill. Image analysis of two-dimensional gels revealed 801 protein spots in the PCT lysate, compared to 760 protein spots in the bead mill lysate.
Authors:
Gary B Smejkal; Myra H Robinson; Nathan P Lawrence; Feng Tao; Calvin A Saravis; Richard T Schumacher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomolecular techniques : JBT     Volume:  17     ISSN:  1524-0215     ISO Abbreviation:  J Biomol Tech     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-06-02     Completed Date:  2006-08-14     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  100888641     Medline TA:  J Biomol Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  173-5     Citation Subset:  IM    
Affiliation:
Pressure BioSciences, Proteomics Laboratory, 6 Gill Street, Woburn, MA 01801, USA. gsmejkal@pressurebiosciences.com
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MeSH Terms
Descriptor/Qualifier:
Biotechnology / methods*
Electrophoresis, Gel, Two-Dimensional / methods
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry*,  isolation & purification*
Image Processing, Computer-Assisted
Oscillometry
Pressure
Proteomics / methods
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins
Comments/Corrections

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