| Increased protein yields from Escherichia coli using pressure-cycling technology. | |
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MedLine Citation:
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PMID: 16741245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sample preparation is critical to the success of two-dimensional gel electrophoresis and other analytical methods. Pressure-cycling technology (PCT) uses alternating cycles of high and low pressure to induce cell lysis. Cell suspensions were placed in PULSE Tubes and subjected to alternating cycles of high and low pressure in a Barocycler instrument. each cycle consisted of 20 sec at 35,000 psi followed by 20 sec at ambient pressure. For the bacterium Escherichia coli, PCT extracted 14.2% more total protein than was extracted using a standard bead mill. Image analysis of two-dimensional gels revealed 801 protein spots in the PCT lysate, compared to 760 protein spots in the bead mill lysate. |
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Authors:
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Gary B Smejkal; Myra H Robinson; Nathan P Lawrence; Feng Tao; Calvin A Saravis; Richard T Schumacher |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomolecular techniques : JBT Volume: 17 ISSN: 1524-0215 ISO Abbreviation: J Biomol Tech Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-06-02 Completed Date: 2006-08-14 Revised Date: 2013-06-07 |
Medline Journal Info:
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Nlm Unique ID: 100888641 Medline TA: J Biomol Tech Country: United States |
Other Details:
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Languages: eng Pagination: 173-5 Citation Subset: IM |
Affiliation:
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Pressure BioSciences, Proteomics Laboratory, 6 Gill Street, Woburn, MA 01801, USA. gsmejkal@pressurebiosciences.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Electrophoresis, Gel, Two-Dimensional / methods Escherichia coli / metabolism* Escherichia coli Proteins / chemistry*, isolation & purification* Image Processing, Computer-Assisted Oscillometry Pressure Proteomics / methods |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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