Document Detail


Increased prosomal proteins in breast cancer cells and in neighboring normal cells in Parsi and non-Parsi populations.
MedLine Citation:
PMID:  9654195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monoclonal antibodies were raised against the prosomal proteins p27K, p29K and the prosome-like protein p21K (PLP) from normal breast glandular cells and from benign and malignant tumors. They were used to clarify the involvement of prosomes in tumorigenesis of human breast cells. Immunostaining showed the distribution of prosomes in the cytoplasm and nuclei of cells from European normal women (EN) and Parsi (P) and non-Parsi (NP) benign (B) and malignant (M) tissues. The flow-cytometry studies showed an increased mean percentage of labeled cells, particularly with anti-p27K prosomal protein mAb, in malignant tissue from NP compared to EN. The p21K data indicated an increase in the number of cells labeled by flow-cytometry studies in all groups compared to EN, while p29K-expressing cells were more abundant in NPN, PB, PM and NPM. Intergroup comparison showed that the mean percentage of cells labeled with anti-p27K and anti-p29K was significantly higher in PB than in NPB, as seen by flow cytometry, whereas there was a higher production or accumulation of the p21K (PLP) prosomal protein in NPM than in PM, as seen by immunostaining. By comparison with EN, there were also significantly more normal cells containing the three antigens in the apparently normal tissue in the neighborhood of the tumor in NPM, and more cells containing p21K in PM patients than in EN. As prosomes are involved in the cell differentiation and in the cell cycle control, the changes observed in breast tissues may be related to oncogenic processes. Furthermore, the modified subunit pattern of prosomes in cancer and, possibly, pre-cancer tissue may be of interest for diagnosis purposes.
Authors:
A Bhui-Kaur; A Therwath; L Henry; J Chiesa; A Kurkure; K Scherrer; J P Bureau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  124     ISSN:  0171-5216     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  1998  
Date Detail:
Created Date:  1998-07-09     Completed Date:  1998-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire et Cytogénétique Moléculaire (UPRES-JE 1952), Faculté de Médecine de Montpellier-Nîmes, Université Montpellier I, Nîmes, France.
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MeSH Terms
Descriptor/Qualifier:
Breast / metabolism*
Breast Neoplasms / metabolism*
Cell Cycle
Cell Nucleus / metabolism
Cysteine Endopeptidases / metabolism*
Cytoplasm / metabolism
Ethnic Groups
Europe / epidemiology
Female
Flow Cytometry
Humans
Immunoenzyme Techniques
India / epidemiology
Ki-67 Antigen / metabolism
Multienzyme Complexes / metabolism*
Proteasome Endopeptidase Complex
RNA-Binding Proteins / metabolism
Chemical
Reg. No./Substance:
0/Ki-67 Antigen; 0/Multienzyme Complexes; 0/RNA-Binding Proteins; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.25.1/PSMA6 protein, human; EC 3.4.25.1/Proteasome Endopeptidase Complex

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