| Increased population of nonhormone-producing cells suggests the presence of dysfunctional growth hormone cells in the anterior pituitary gland of the spontaneous dwarf rat. | |
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MedLine Citation:
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PMID: 8389999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Anterior pituitary gland of the spontaneous dwarf rat (SDR) with isolated growth hormone (GH) deficiency was studied using immunocytochemistry, cell count and in situ hybridization. The standard immunocytochemistry of five anterior pituitary hormones [adrenocorticotropic hormone (ACTH), luteinizing hormone, thyroid-stimulating hormone, prolactin (PRL) and GH] and S-100 protein failed to detect any cytological difference between normal rats and SDRs, except for the size of different types of cells which were smaller in SDR than in normal rats, and GH cells which were undetectable in the SDR. The cell count study again showed lack of immunoreactive GH cells in the SDR. The population of PRL cells was significantly reduced in the SDR by 40% in male and 30% in female when compared to those of the control. The population of ACTH cells was larger in the male SDR. The population of the immunonegative cells was calculated by subtracting the sum of the percentages of immunopositive cells from 100, and it was found to be remarkably increased in the SDR. The population of immunonegative cells was about 55% in both male and female SDRs, whereas it was calculated to be 18.7% (male) or 10.2% (female) in the control rats. In situ hybridization study using GH cRNA indicated the presence of a considerable number of cells which express GH mRNA in the SDR as well as in the control rats. These results taken as a whole suggest the presence of a number of dysfunctional GH cells in the SDR.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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H Nogami; T Takeuchi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroendocrinology Volume: 57 ISSN: 0028-3835 ISO Abbreviation: Neuroendocrinology Publication Date: 1993 |
Date Detail:
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Created Date: 1993-07-12 Completed Date: 1993-07-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0035665 Medline TA: Neuroendocrinology Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 374-80 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenocorticotropic Hormone
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analysis Animals Cell Count Dwarfism, Pituitary / metabolism, pathology* Female Growth Hormone / analysis, deficiency*, genetics Immunohistochemistry In Situ Hybridization Male Pituitary Gland, Anterior / pathology* Pituitary Hormones, Anterior / analysis RNA, Messenger / analysis Rats S100 Proteins / analysis Thyrotropin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Pituitary Hormones, Anterior; 0/RNA, Messenger; 0/S100 Proteins; 9002-60-2/Adrenocorticotropic Hormone; 9002-71-5/Thyrotropin; 9002-72-6/Growth Hormone |
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