Document Detail


Increased polyploidy in aortic vascular smooth muscle cells during aging is marked by cellular senescence.
MedLine Citation:
PMID:  17291294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously reported that the frequency of polyploid aortic vascular smooth muscle cells (VSMC) serves as a biomarker of aging. Cellular senescence of somatic cells is another marker of aging that is characterized by the inability to undergo cell division. Here, we examined whether polyploidy is associated with the development of cellular senescence in vivo. Analysis of aortic tissue preparations from young and old Brown Norway rats showed that expression of senescence markers such as p16(INK4a) and senescence-associated beta-galactosidase activity are detected primarily in the old tissues. VSMC from p16(INK4a) knockout and control mice display similar levels of polyploid cells. Intriguingly, senescence markers are expressed in most, but not all, polyploid VSMC. Moreover, the polyploid cells exhibit limited proliferative capacity in comparison to their diploid counterparts. This study is the first to demonstrate in vivo that polyploid VSMC adopt a senescent phenotype.
Authors:
Dan Yang; Donald J McCrann; Hao Nguyen; Cynthia St Hilaire; Ronald A DePinho; Matthew R Jones; Katya Ravid
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-02-07
Journal Detail:
Title:  Aging cell     Volume:  6     ISSN:  1474-9718     ISO Abbreviation:  Aging Cell     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-22     Completed Date:  2007-06-07     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  101130839     Medline TA:  Aging Cell     Country:  England    
Other Details:
Languages:  eng     Pagination:  257-60     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / cytology
Biological Markers / metabolism
Cell Aging*
Microscopy, Phase-Contrast
Muscle, Smooth, Vascular / cytology*
Myocytes, Smooth Muscle / cytology,  metabolism*
Phenotype
Polyploidy*
Rats
Grant Support
ID/Acronym/Agency:
AG022623/AG/NIA NIH HHS; R03 AG022623-01/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers
Comments/Corrections

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