| Increased pneumotoxicity of lipopolysaccharide from E.coli in nitric oxide deficient blood-perfused rat lungs. | |
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MedLine Citation:
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PMID: 9444614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated lungs of Wistar rats were ventilated by air that was enriched with 5% CO2 and perfused with homologous blood in a closed circuit (9.5 +/- 1 ml/min) using Hugo Sachs apparatus type 829. Lipopolysaccharide from E.coli (LPS, serotype 0127:B8) at a selected sub-toxic concentration of 300 micrograms/ml added to recirculating blood produced a biphasic response. Instant transient increase in pulmonary arterial and venous perfusion pressures, and a decrease in air tidal volume, and fifty minutes later slowly progressing decrease in air tidal volume without changes in pulmonary haemodynamics, were observed. Inhibition of pulmonary nitric oxide synthase by instillation of NG-nitro-L-arginine methyl ester (L-NAME) at a final concentration of 300 microM to recirculating blood dramatically changed the response to LPS. In nitric oxide deficient lungs LPS caused prompt increase in arterial and venous pressures and a fall in air tidal volume with accompanying rise in airway resistance. Within 6.3 +/- 0.5 min a fulminant pulmonary oedema developed and all functions of the lung stopped abruptly. We conclude that pulmonary nitric oxide plays a defensive role in protecting rat lungs against LPS-induced injury. |
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Authors:
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J B Bartuś; S Chłopicki; R J Gryglewski |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society Volume: 48 ISSN: 0867-5910 ISO Abbreviation: J. Physiol. Pharmacol. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-03-03 Completed Date: 1998-03-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9114501 Medline TA: J Physiol Pharmacol Country: POLAND |
Other Details:
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Languages: eng Pagination: 655-63 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Jagiellonian University Medical College, Cracow, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure Escherichia coli* Female Lipopolysaccharides / toxicity* Lung Diseases / chemically induced*, physiopathology NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / antagonists & inhibitors* Perfusion Rats Rats, Wistar Tidal Volume |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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