Document Detail


Increased pneumotoxicity of lipopolysaccharide from E.coli in nitric oxide deficient blood-perfused rat lungs.
MedLine Citation:
PMID:  9444614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolated lungs of Wistar rats were ventilated by air that was enriched with 5% CO2 and perfused with homologous blood in a closed circuit (9.5 +/- 1 ml/min) using Hugo Sachs apparatus type 829. Lipopolysaccharide from E.coli (LPS, serotype 0127:B8) at a selected sub-toxic concentration of 300 micrograms/ml added to recirculating blood produced a biphasic response. Instant transient increase in pulmonary arterial and venous perfusion pressures, and a decrease in air tidal volume, and fifty minutes later slowly progressing decrease in air tidal volume without changes in pulmonary haemodynamics, were observed. Inhibition of pulmonary nitric oxide synthase by instillation of NG-nitro-L-arginine methyl ester (L-NAME) at a final concentration of 300 microM to recirculating blood dramatically changed the response to LPS. In nitric oxide deficient lungs LPS caused prompt increase in arterial and venous pressures and a fall in air tidal volume with accompanying rise in airway resistance. Within 6.3 +/- 0.5 min a fulminant pulmonary oedema developed and all functions of the lung stopped abruptly. We conclude that pulmonary nitric oxide plays a defensive role in protecting rat lungs against LPS-induced injury.
Authors:
J B Bartuś; S Chłopicki; R J Gryglewski
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of physiology and pharmacology : an official journal of the Polish Physiological Society     Volume:  48     ISSN:  0867-5910     ISO Abbreviation:  J. Physiol. Pharmacol.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-03-03     Completed Date:  1998-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9114501     Medline TA:  J Physiol Pharmacol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  655-63     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Jagiellonian University Medical College, Cracow, Poland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure
Escherichia coli*
Female
Lipopolysaccharides / toxicity*
Lung Diseases / chemically induced*,  physiopathology
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors*
Perfusion
Rats
Rats, Wistar
Tidal Volume
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase

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