| Increased plasmin and serine proteinase activity during flow-induced intimal atrophy in baboon PTFE grafts. | |
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MedLine Citation:
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PMID: 11884281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High blood flow causes intimal atrophy and loss of extracellular matrix in PTFE aortoiliac grafts. We have investigated whether matrix-degrading proteinases are altered in this baboon model of atrophy using zymography, western analysis, and a versican degradation assay. After four days of high flow, urokinase was increased and plasminogen activator inhibitor-1 was decreased in the intima. Plasminogen was increased after seven days. Pro-matrix metalloproteinase (MMP)-2, activated MMP-2, and proMMP-9 levels were modestly increased by high flow at 7 days, whereas MMP-3 and tissue inhibitor of metalloproteinases-1 were not altered. Extracts of 4-day high-flow intimas degraded more 35S-methionine-labeled versican than low-flow intimal extracts, and this activity was inhibited by AEBSF, a serine proteinase inhibitor, and a plasmin antibody. In contrast, this activity was not inhibited by the MMP inhibitor, BB-94 (Batimastat). These data suggest that serine proteinases, including plasmin, may be largely responsible for extracellular matrix degradation in this primate model of flow-induced intimal atrophy. |
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Authors:
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Richard D Kenagy; Jens W Fischer; Mark G Davies; Scott A Berceli; Suzanne M Hawkins; Thomas N Wight; Alexander W Clowes |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 22 ISSN: 1524-4636 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-03-08 Completed Date: 2002-03-14 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 400-4 Citation Subset: IM |
Affiliation:
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Division of Vascular Surgery, Department of Surgery, University of Washington, Seattle 98195-6410, USA. rkenagy@u.washington.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrophy Blood Vessel Prosthesis* Fibrinolysin / metabolism Iliac Artery Lectins, C-Type Male Matrix Metalloproteinases / metabolism Papio Peripheral Vascular Diseases / enzymology*, etiology, pathology Plasminogen Activators / metabolism Polytetrafluoroethylene* Protease Inhibitors / metabolism Proteochondroitin Sulfates / metabolism Regional Blood Flow Serine Endopeptidases / metabolism* Tunica Intima* / pathology Urokinase-Type Plasminogen Activator / metabolism Versicans |
| Grant Support | |
ID/Acronym/Agency:
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HL07828/HL/NHLBI NIH HHS; HL18645/HL/NHLBI NIH HHS; HL30946/HL/NHLBI NIH HHS; RR00166/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lectins, C-Type; 0/Protease Inhibitors; 0/Proteochondroitin Sulfates; 126968-45-4/Versicans; 9002-84-0/Polytetrafluoroethylene; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.7/Fibrinolysin; EC 3.4.21.73/Urokinase-Type Plasminogen Activator; EC 3.4.24.-/Matrix Metalloproteinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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