| Increased plasma P-selectin induced by intravenous administration of endotoxin in rats. | |
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MedLine Citation:
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PMID: 9610374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gelfiltration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat. |
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Authors:
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E Misugi; S J Tojo; T Yasuda; Y Kurata; S Morooka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 246 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-06-15 Completed Date: 1998-06-15 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 414-7 Citation Subset: IM |
Affiliation:
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Sumitomo Pharmaceuticals Research Center, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding, Competitive Disease Models, Animal Enzyme-Linked Immunosorbent Assay / methods*, standards Injections, Intravenous Kinetics Lipopolysaccharides / administration & dosage, toxicity* Male P-Selectin / biosynthesis, blood* Rats Rats, Sprague-Dawley Reference Standards Solubility |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/P-Selectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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