| Increased phosphorylated p38 mitogen activated protein kinase in COPD lungs. | |
| | |
MedLine Citation:
|
PMID: 23060629 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The p38 Mitogen Activated Protein Kinase (MAPK) pathway is upregulated in COPD. To date dual-labelling to identify cell-type specific presence of phosphorylated (phospho-) p38 MAPK has not been carried out.Phospho-p38 MAPK was quantified in a variety of cell types in the lung tissue of 20 COPD patients, 12 smokers and 12 non-smokers using immunohistochemistry. Paired blood and sputum neutrophils (n=7 COPD), and CD8 and epithelial cells (n=3 COPD) were cultured with a p38 MAPK inhibitor. Supernatant TNFα, CXCL8 levels were analysed by ELISA. Sputum and blood neutrophil cytospins were analysed for phospho-p38 MAPK.Phospho-p38 MAPK was increased in bronchial epithelial cells, macrophages and CD20+and CD8+lymphocytes in COPD lungs. Sputum and lung tissue neutrophils were devoid of phospho-p38 in all patient groups. The p38 MAPK inhibitor SB100 attenuated pro-inflammatory mediator release in COPD lung CD8 cells and airway epithelia but there was no effect on COPD sputum neutrophils. Our data indicates cell specific anti-inflammatory effects of p38 MAPK inhibition in the lung. |
| | |
Authors:
|
Kate Gaffey; Sophie Reynolds; Jonathan Plumb; Manminder Kaur; Dave Singh |
Related Documents
:
|
12765939 - Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphoryl... 1649189 - Hormone-sensitive cyclic gmp-inhibited cyclic amp phosphodiesterase in rat adipocytes. ... 17052199 - Regulation by per-arnt-sim (pas) kinase of pancreatic duodenal homeobox-1 nuclear impor... 15870689 - Interaction between brk kinase and insulin receptor substrate-4. 18460599 - Acute dexamethasone-induced increase in cardiac lipoprotein lipase requires activation ... 11737239 - Surfing the insulin signaling web. 8588739 - Molecular mechanisms of endotoxin activity. 11897489 - Nitric oxide and the other cyclic nucleotide. 18194429 - The effect of amp-activated kinase activation on gonadotrophin-releasing hormone secret... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-11 |
Journal Detail:
|
Title: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology Volume: - ISSN: 1399-3003 ISO Abbreviation: Eur. Respir. J. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8803460 Medline TA: Eur Respir J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
NIHR Translational Research Facility, University Hospital of South Manchester. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of montelukast on respiratory symptoms and lung function in wheezy infants.
Next Document: The use of inhaled corticosteroids and the risk of adrenal insufficiency.