Document Detail


Increased phosphorylated p38 mitogen activated protein kinase in COPD lungs.
MedLine Citation:
PMID:  23060629     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The p38 Mitogen Activated Protein Kinase (MAPK) pathway is upregulated in COPD. To date dual-labelling to identify cell-type specific presence of phosphorylated (phospho-) p38 MAPK has not been carried out.Phospho-p38 MAPK was quantified in a variety of cell types in the lung tissue of 20 COPD patients, 12 smokers and 12 non-smokers using immunohistochemistry. Paired blood and sputum neutrophils (n=7 COPD), and CD8 and epithelial cells (n=3 COPD) were cultured with a p38 MAPK inhibitor. Supernatant TNFα, CXCL8 levels were analysed by ELISA. Sputum and blood neutrophil cytospins were analysed for phospho-p38 MAPK.Phospho-p38 MAPK was increased in bronchial epithelial cells, macrophages and CD20+and CD8+lymphocytes in COPD lungs. Sputum and lung tissue neutrophils were devoid of phospho-p38 in all patient groups. The p38 MAPK inhibitor SB100 attenuated pro-inflammatory mediator release in COPD lung CD8 cells and airway epithelia but there was no effect on COPD sputum neutrophils. Our data indicates cell specific anti-inflammatory effects of p38 MAPK inhibition in the lung.
Authors:
Kate Gaffey; Sophie Reynolds; Jonathan Plumb; Manminder Kaur; Dave Singh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-11
Journal Detail:
Title:  The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology     Volume:  -     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
NIHR Translational Research Facility, University Hospital of South Manchester.
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