Document Detail


Increased perfusion pressure enhances the expression of endothelin (ETB) and angiotensin II (AT1, AT2) receptors in rat mesenteric artery smooth muscle cells.
MedLine Citation:
PMID:  19353416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, we hypothesized that changes in perfusion pressure result in altered expression of mRNA and protein encoding for the ETA-, ETB-, AT1- and AT2-receptors in rat mesenteric vessels. Segments of the rat mesenteric artery were cannulated with glass micropipettes, pressurized and luminally perfused in a perfusion chamber. After either exposure to no ("organ culture" (0 mmHg)), normal (85/75 mmHg) or high pressure (160/150 mmHg) at constant flow for 1-17 h, the vessel segments were snap frozen and real-time polymerase chain reaction was performed to quantify the ET- and AT-receptor mRNA content, or immersed in a fixative solution, dehydrated, frozen, cut in a cryostat and immunohistology stained for ET- and AT-receptor protein. The mRNA expressions of ETB and of AT2 were significantly enhanced in vessels exposed to high perfusion pressure, compared with normal and no perfusion pressure at 4 h. In concordance, AT1-, AT2- and ETB-receptor proteins were up-regulated at 17 h of high perfusion pressure. In conclusion, the results from our rat perfusion model suggest a more important role of shear stress than pure pressure alone and may serve as a surrogate model for studies designed to investigate hypertension mechanisms.
Authors:
Isak Lindstedt; Cang-Bao Xu; Yaping Zhang; Lars Edvinsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood pressure     Volume:  18     ISSN:  1651-1999     ISO Abbreviation:  Blood Press.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-08     Completed Date:  2009-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301454     Medline TA:  Blood Press     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  78-85     Citation Subset:  IM    
Affiliation:
Division of Experimental Vascular Research, Institute of Clinical Science in Lund, Lund University, Lund, Sweden. isaklindstedt@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / physiology*
Gene Expression Regulation / physiology*
Mesenteric Arteries / metabolism,  physiology*
Myocytes, Smooth Muscle / metabolism*
Perfusion
Pulsatile Flow / physiology*
RNA Stability
RNA, Messenger / biosynthesis,  genetics
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1 / biosynthesis*,  genetics
Receptor, Angiotensin, Type 2 / biosynthesis*,  genetics
Receptor, Endothelin A / biosynthesis*,  genetics
Receptor, Endothelin B / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/Receptor, Angiotensin, Type 2; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B

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