| Increased perfusion pressure enhances the expression of endothelin (ETB) and angiotensin II (AT1, AT2) receptors in rat mesenteric artery smooth muscle cells. | |
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MedLine Citation:
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PMID: 19353416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we hypothesized that changes in perfusion pressure result in altered expression of mRNA and protein encoding for the ETA-, ETB-, AT1- and AT2-receptors in rat mesenteric vessels. Segments of the rat mesenteric artery were cannulated with glass micropipettes, pressurized and luminally perfused in a perfusion chamber. After either exposure to no ("organ culture" (0 mmHg)), normal (85/75 mmHg) or high pressure (160/150 mmHg) at constant flow for 1-17 h, the vessel segments were snap frozen and real-time polymerase chain reaction was performed to quantify the ET- and AT-receptor mRNA content, or immersed in a fixative solution, dehydrated, frozen, cut in a cryostat and immunohistology stained for ET- and AT-receptor protein. The mRNA expressions of ETB and of AT2 were significantly enhanced in vessels exposed to high perfusion pressure, compared with normal and no perfusion pressure at 4 h. In concordance, AT1-, AT2- and ETB-receptor proteins were up-regulated at 17 h of high perfusion pressure. In conclusion, the results from our rat perfusion model suggest a more important role of shear stress than pure pressure alone and may serve as a surrogate model for studies designed to investigate hypertension mechanisms. |
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Authors:
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Isak Lindstedt; Cang-Bao Xu; Yaping Zhang; Lars Edvinsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Blood pressure Volume: 18 ISSN: 1651-1999 ISO Abbreviation: Blood Press. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-04-08 Completed Date: 2009-07-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9301454 Medline TA: Blood Press Country: Norway |
Other Details:
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Languages: eng Pagination: 78-85 Citation Subset: IM |
Affiliation:
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Division of Experimental Vascular Research, Institute of Clinical Science in Lund, Lund University, Lund, Sweden. isaklindstedt@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology* Gene Expression Regulation / physiology* Mesenteric Arteries / metabolism, physiology* Myocytes, Smooth Muscle / metabolism* Perfusion Pulsatile Flow / physiology* RNA Stability RNA, Messenger / biosynthesis, genetics Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 / biosynthesis*, genetics Receptor, Angiotensin, Type 2 / biosynthesis*, genetics Receptor, Endothelin A / biosynthesis*, genetics Receptor, Endothelin B / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/Receptor, Angiotensin, Type 2; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B |
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