| Increased perfusion and angiogenesis in a hindlimb ischemia model with plasmid FGF-2 delivered by noninvasive electroporation. | |
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MedLine Citation:
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PMID: 20393507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gene therapy approaches delivering fibroblast growth factor-2 (FGF-2) have shown promise as a potential treatment for increasing blood flow to ischemic limbs. Currently, effective noninvasive techniques to deliver plasmids encoding genes of therapeutic interest, such as FGF-2, are limited. We sought to determine if intradermal injection of plasmid DNA encoding FGF-2 (pFGF) followed by noninvasive cutaneous electroporation (pFGFE+) could increase blood flow and angiogenesis in a rat model of hindlimb ischemia. pFGFE+ or control treatments were administered on postoperative day 0. Compared to injection of pFGF alone (pFGFE-), delivery of pFGFE+ significantly increased FGF-2 expression for 10 days. Further, the increase in FGF-2 expression with pFGFE+ was sufficient to significantly increase ischemic limb blood flow, measured by laser Doppler perfusion imaging, beginning on postoperative day 3. Ischemic limb blood flow in the pFGFE+ treatment group remained significantly higher than all control groups through the end point of the study, postoperative day 14. Immunohistochemical staining of gastrocnemius cross sections determined there was a twofold increase in capillary density in the pFGFE+ treatment group. Our results suggest that pFGFE+ is a potential noninvasive, nonviral therapeutic approach to increase perfusion and angiogenesis for the treatment of limb ischemia. |
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Authors:
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B Ferraro; Y L Cruz; M Baldwin; D Coppola; R Heller |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-04-15 |
Journal Detail:
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Title: Gene therapy Volume: 17 ISSN: 1476-5462 ISO Abbreviation: Gene Ther. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-09-27 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 9421525 Medline TA: Gene Ther Country: England |
Other Details:
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Languages: eng Pagination: 763-9 Citation Subset: IM |
Affiliation:
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Department of Molecular Medicine, University of South Florida, Tampa, FL, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Electroporation Fibroblast Growth Factor 2 / biosynthesis, genetics* Gene Therapy / methods* Gene Transfer Techniques Hindlimb / blood supply* Ischemia / therapy* Neovascularization, Physiologic Peripheral Vascular Diseases / therapy Rats Regional Blood Flow |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB005441-03/EB/NIBIB NIH HHS; R01 EB005441-04/EB/NIBIB NIH HHS; R01 EB005441-05/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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103107-01-3/Fibroblast Growth Factor 2 |
| Comments/Corrections | |
Comment In:
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Gene Ther. 2010 Jun;17(6):691
[PMID:
20463758
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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