Document Detail


Increased oxidative activity in human blood neutrophils and monocytes after spinal cord injury.
MedLine Citation:
PMID:  19056384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Traumatic injury can cause a systemic inflammatory response, increasing oxidative activity of circulating leukocytes and potentially exacerbating the original injury, as well as causing damage to initially unaffected organs. Although the importance of intraspinal inflammation after human spinal cord injury is appreciated, the role of the systemic inflammatory response to this injury is not widely recognised. We investigated oxidative activity of blood leukocytes from nine cord-injured subjects and six trauma controls (bone fractures without CNS injury) at 6 h-2 weeks after injury, comparing values to those of ten uninjured subjects. Neutrophil and monocyte free radical production, evaluated by flow cytometry, increased significantly more in cord injury subjects than in trauma controls (6-fold vs 50% increases). In leukocyte homogenates, the concentration of free radicals increased significantly more in cord injury subjects (2-fold) than in the trauma controls (1.6-fold) as did activity of myeloperoxidase (2.3-fold vs. 1.7-fold). Moreover, in homogenates and blood smears, expression of the NADPH oxidase subunit gp91(phox) and of the oxidative enzyme, inducible nitric oxide synthetase was 20-25% greater in cord injury subjects than in trauma controls. Expression of the pro-inflammatory transcription factor NF-kappaB and of cyclooxygenase-2 increased similarly after both injuries. Finally, aldehyde products of tissue-damaging lipid peroxidation also increased significantly more in the plasma of spinal cord injury subjects than in trauma controls (2.6 fold vs. 1.9-fold). Spinal cord injury causes a particularly intense systemic inflammatory response. Limiting this response briefly after cord injury should protect the spinal cord and tissues/organs outside the CNS from secondary damage.
Authors:
Feng Bao; Christopher S Bailey; Kevin R Gurr; Stewart I Bailey; M Patricia Rosas-Arellano; Gregory A Dekaban; Lynne C Weaver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-13
Journal Detail:
Title:  Experimental neurology     Volume:  215     ISSN:  1090-2430     ISO Abbreviation:  Exp. Neurol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-22     Completed Date:  2009-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  308-16     Citation Subset:  IM    
Affiliation:
Spinal Cord Injury Team, BioTherapeutics Research Group, Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, 100 Perth Drive, London, ON, Canada N6A 5K8.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged, 80 and over
Analysis of Variance
Cyclooxygenase 2 / metabolism
Female
Fluoresceins / diagnostic use
Humans
Lipid Peroxidation / physiology
Male
Middle Aged
Monocytes / metabolism*
NF-kappa B / metabolism
Neutrophils / metabolism*
Nitric Oxide Synthase Type II / metabolism
Peroxidase
Reactive Oxygen Species / blood*
Rhodamines / diagnostic use
Spinal Cord Injuries / blood*
Young Adult
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/NF-kappa B; 0/Reactive Oxygen Species; 0/Rhodamines; 109244-58-8/dihydrorhodamine 123; 2044-85-1/diacetyldichlorofluorescein; EC 1.11.1.7/Peroxidase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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