| Increased neutrophil oxidative burst metabolism in systemic lupus erythematosus. | |
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MedLine Citation:
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PMID: 23012249 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Introduction: There is increased frequency of discoid lesions (2.7%) and SLE (0.5%) in patients with chronic granulomatosus disease, but the literature is still controversial about phagocyte oxidative burst in SLE patients. Materials and methods: 300 SLE patients and 301 blood donors were evaluated for quantitation of the oxidative burst in phagocytes by flow cytometry based on the oxidation of 2,7-dichlorofluorescein-diacetate after stimuli with Staphylococcus aureus and Pseudomonas aeruginosa. Results: Neutrophils from SLE patients displayed higher basal reactive oxygen species (ROS) production than healthy controls [Mean of fluorescence intensity (MFI) = 53.77 ± 11.38 vs 15.08 ± 2.63, p < 0.001] and after stimulation with S. aureus (MFI = 355.46 ± 58.55 vs 151.92 ± 28.25, p < 0.001) or P. aeruginosa (MFI = 82.53 ± 10.1 vs 48.99 ± 6.74, p < 0.001). There was stronger neutrophil response after bacterial stimuli (ΔMFI) in SLE patients than in healthy controls (S. aureus = 301.69 ± 54.42 vs 118.38 ± 26.03, p < 0.001; P. aeruginosa = 28.76 ± 12.3 vs 15.45 ± 5.15, p < 0.001), but no difference with respect to the oxidative burst profile according to disease activity (SLEDAI ≥ 6) or severity (SLICC-DI ≥2). Patients with kidney involvement presented higher basal and stimulated ROS production in neutrophils. Discussion: The present findings corroborate the important role of innate immunity in SLE and implicate neutrophils in the pathophysiology of the disease. |
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Authors:
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S F Perazzio; R Salomao; N P Silva; L E C Andrade |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-25 |
Journal Detail:
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Title: Lupus Volume: - ISSN: 1477-0962 ISO Abbreviation: Lupus Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9204265 Medline TA: Lupus Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Division of Rheumatology, UNIFESP/EPM, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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