| Increased neonatal mortality in mice lacking cellular retinol-binding protein II. | |
| | |
MedLine Citation:
|
PMID: 12138113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cellular retinol-binding protein II (CRBP II) is a member of the cellular retinol-binding protein family, which is expressed primarily in the small intestine. To investigate the physiological role of CRBP II, the gene encoding CRBP II was inactivated. The saturable component of intestinal retinol uptake is impaired in CRBP II(-/-) mice. The knockout mice, while maintained on a vitamin A-enriched diet, have reduced (40%) hepatic vitamin A stores but grow and reproduce normally. However, reducing maternal dietary vitamin A to marginal levels during the latter half of gestation results in 100% mortality/litter within 24 h after birth in the CRBP II(-/-) line but no mortality in the wild type line. The neonatal mortality in heterozygote offspring of CRBP II(-/-) dams (79 +/- 21% deaths/litter) was increased as compared with the neonatal mortality in heterozygote offspring of wild type dams (29 +/- 25% deaths per litter, p < 0.05). Maternal CRBP II was localized by immunostaining in the placenta at 18 days postcoitum as well as in the small intestine. These studies suggest that both fetal as well as maternal CRBP II are required to ensure adequate delivery of vitamin A to the developing fetus when dietary vitamin A is limiting. |
| | |
Authors:
|
Xueping E; Liang Zhang; Jianyun Lu; Patrick Tso; William S Blaner; Marc S Levin; Ellen Li |
Related Documents
:
|
20889523 - Respiratory outcomes in early childhood following antenatal vitamin c and e supplementa... 20511053 - Maternal vitamin d status: implications for the development of infantile nutritional ri... 11978753 - Low levels of pancreatic elastase 1 in stools of preterm infants. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2002-07-22 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 277 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2002 Sep |
Date Detail:
|
Created Date: 2002-09-23 Completed Date: 2002-11-13 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 36617-23 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Washington University, St. Louis School of Medicine, St. Louis, Missouri 63110, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA, Complementary / metabolism Female Genetic Vectors Heterozygote Immunohistochemistry Intestine, Small / metabolism Liver / metabolism Male Maternal-Fetal Exchange Mice Mice, Knockout Models, Genetic Placenta / metabolism Pregnancy Protein Binding RNA / metabolism Retinoids / metabolism Retinol-Binding Proteins / genetics*, physiology* Retinol-Binding Proteins, Cellular Time Factors Vitamin A / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
DK 52574/DK/NIDDK NIH HHS; DK 56341/DK/NIDDK NIH HHS; DK40172/DK/NIDDK NIH HHS; DK50446/DK/NIDDK NIH HHS; DK52444/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/Rbp2 protein, mouse; 0/Retinoids; 0/Retinol-Binding Proteins; 0/Retinol-Binding Proteins, Cellular; 11103-57-4/Vitamin A; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of iron nitrosylation on sickle cell hemoglobin solubility.
Next Document: Crystal structure of the Apo, unactivated insulin-like growth factor-1 receptor kinase. Implication ...