| Increased myocardial matrix metalloproteinases in hypoxic newborn pigs during resuscitation: effects of oxygen and carbon dioxide. | |
| | |
MedLine Citation:
|
PMID: 15255782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Perinatal asphyxia is associated with cardiac dysfunction, and it is important to prevent further tissue injury during resuscitation. There is increasing evidence that myocardial matrix metalloproteinases (MMPs) are involved in myocardial hypoxaemia-reoxygenation injury. OBJECTIVE: To assess MMPs and antioxidant capacity in newborn pigs after global ischaemia and subsequent resuscitation with ambient air or 100% O(2) at different PaCO(2)-levels. METHODS: Newborn pigs (12-36 h of age) were resuscitated for 30 min by ventilation with 21% or 100% O(2) at different PaCO(2) levels after a hypoxic insult, and thereafter observed for 150 min. In myocardial tissue extracts, MMPs were analyzed by gelatin zymography and broad matrix-degrading capacity (total MMP). Total endogenous antioxidant capacity in myocardial tissue extracts was measured by the oxygen radical absorbance capacity (ORAC) assay. RESULTS: Matrix metalloproteinase-2 more than doubled from baseline values (P < 0.001), and was higher in piglets resuscitated with 100% O(2) than with ambient air (P = 0.012). The ORAC value was considerably decreased (P < 0.001). In piglets with elevated PaCO(2), total MMP-activity in the right ventricle was more increased than in the left ventricle (P = 0.008). In the left ventricle, total MMPactivity was higher in the piglets with low PaCO(2) than in the piglets with elevated PaCO(2) (P = 0.013). CONCLUSION: In hypoxaemia-reoxygenation injury the MMP-2 level was highly increased and was most elevated in the piglets resuscitated with 100% O(2). Antioxidant capacity was considerably decreased. Assessed by total MMP-activity, elevated PaCO(2) during resuscitation might protect the left ventricle, and probably increase right ventricle injury of the myocardium. |
| | |
Authors:
|
W B Borke; B H Munkeby; B Halvorsen; K Bjornland; S H Tunheim; G I A Borge; E Thaulow; O D Saugstad |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of clinical investigation Volume: 34 ISSN: 0014-2972 ISO Abbreviation: Eur. J. Clin. Invest. Publication Date: 2004 Jul |
Date Detail:
|
Created Date: 2004-07-16 Completed Date: 2004-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0245331 Medline TA: Eur J Clin Invest Country: England |
Other Details:
|
Languages: eng Pagination: 459-66 Citation Subset: IM |
Affiliation:
|
Rikshospitalet University Hospital, Oslo, Norway. w.b.borke@klinmed.uio.no |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Anoxia / enzymology* Antioxidants / metabolism Carbon Dioxide / blood* Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinases / metabolism* Myocardium / enzymology* Oxygen / physiology* Resuscitation Reverse Transcriptase Polymerase Chain Reaction / methods Swine |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 124-38-9/Carbon Dioxide; 7782-44-7/Oxygen; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 8-isoprostane increases scavenger receptor A and matrix metalloproteinase activity in THP-1 macropha...
Next Document: HDL metabolic activities in a boy with lipoprotein lipase deficiency and his family.