Document Detail


Increased migration of a human glioma cell line after in vitro Cyberknife irradiation.
MedLine Citation:
PMID:  21775821     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8Gy was delivered intermittently over a period of 40 minutes. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF-B at four days (Real time PCR), β1-integrin at 4-5 days (real-time PCR and western blot) and no elevation in phosphorylated AKT at days 4 and 5 (western blot). Our data suggests that glioma cell invasion as well as elevations of TGF-B and β1-integrin are associated with IIR and not CIR.
Authors:
Alessandra Canazza; Chiara Calatozzolo; Luisa Fumagalli; Achille Bergantin; Francesco Ghielmetti; Laura Fariselli; Danilo Croci; Andrea Salmaggi; Emilio Ciusani
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-01
Journal Detail:
Title:  Cancer biology & therapy     Volume:  12     ISSN:  1555-8576     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-7-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Fondazione IRCCS Istituto Neurologico "C. Besta", Milano, Italy.
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