| Increased microsomal oxidation of alcohols after pyrazole treatment and its similarities to the induction by ethanol consumption. | |
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MedLine Citation:
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PMID: 6466709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microsomes isolated from rats treated for 3 days with 200 mg/kg body wt. per day of pyrazole, a potent inhibitor of alcohol dehydrogenase, catalyzed the oxidation of ethanol and 2-butanol at rates 2-3-fold higher than saline controls. This increase was blocked by carbon monoxide, and was not associated with an increase in the oxidation of aminopyrine or in the content of cytochrome P-450, suggesting the possibility of an induction of an alcohol-preferring cytochrome P-450 by pyrazole. Microsomes from the pyrazole-treated rats displayed a stereochemical preference for the oxidation of the (+)-2-butanol isomer over the (-)-2-butanol isomer, which was blocked by carbon monoxide, and also displayed a type-2 binding spectrum with dimethylsulfoxide or 2-butanol. No such spectrum was found with the saline controls. These properties are similar to those which are observed with microsomes from chronic ethanol-fed rats. These similarities suggest the possibility that pyrazole treatment may induce a cytochrome P-450 isozyme with properties similar to the ethanol-inducible cytochrome P-450. |
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Authors:
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G Krikun; A I Cederbaum |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 801 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1984-10-25 Completed Date: 1984-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 131-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Butanol Alcohols / metabolism* Aminopyrine / metabolism Animals Butanols / metabolism Cytochrome P-450 Enzyme System / metabolism Ethanol / metabolism, pharmacology* Kinetics Male Microsomes, Liver / drug effects, metabolism* Oxidation-Reduction Pyrazoles / pharmacology* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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2K02-AA-00003/AA/NIAAA NIH HHS; AA03312/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alcohols; 0/Butanols; 0/Pyrazoles; 288-13-1/pyrazole; 58-15-1/Aminopyrine; 64-17-5/Ethanol; 71-36-3/1-Butanol; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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