| Increased membrane sphingomyelin and arachidonic acid in stroke-prone spontaneously hypertensive rats. | |
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MedLine Citation:
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PMID: 11724215 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cell membrane composition and fluidity are altered in hypertension. Previous reports suggest arachidonic acid, a metabolically active fatty acid, is increased in the membranes of hypertensive animals compared to control. This increase in unsaturated fatty acids does not explain the observed reduction in fluidity in hypertensive rats, suggesting some other factors affecting fluidity may be present. It has been suggested that the metabolism of sphingomyelin is altered in genetic hypertension. We hypothesized that membrane sphingomyelin content is increased in hypertensive animals. PROCEDURES: Stroke-prone spontaneously hypertensive rats (n = 8) were compared with Wistar-Kyoto rats (n = 8). Erythrocyte membranes were prepared and the lipids extracted and separated. Fatty acid methyl esters were produced, identified, and quantified by gas chromatography-mass spectrometry; membrane lipid content was also assessed. RESULTS: The concentration of sphingomyelin was higher in the membrane of the hypertensive rats (45.7+/-6 v 22.4+/-2 microg/mg of protein) compared to control. The previously observed increase in membrane arachidonic acid content was observed in hypertensive animals when compared to control (130+/-32 v 40+/-3 microg/mg of protein). However, this difference was confined to the phosphatidylinositol (18+/-4 v 6.5+/-1.5 microg/mg of protein) and free fatty acid (2.1+/-0.4 v 0.6+/-0.1 microg/mg of protein) fractions. CONCLUSION: We hypothesize that reports of reduced membrane fluidity observed in hypertension may be due to an increase in the proportion of sphingomyelin in the cell membrane. |
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Authors:
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A M Dorrance; D Graham; R C Webb; R Fraser; A Dominiczak |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of hypertension Volume: 14 ISSN: 0895-7061 ISO Abbreviation: Am. J. Hypertens. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-28 Completed Date: 2002-03-06 Revised Date: 2009-02-24 |
Medline Journal Info:
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Nlm Unique ID: 8803676 Medline TA: Am J Hypertens Country: United States |
Other Details:
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Languages: eng Pagination: 1149-53 Citation Subset: IM |
Affiliation:
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Department of Medicine and Therapeutics, University of Glasgow, Western Infirmary, Scotland. dorrance@psych.mcg.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism* Cell Membrane / metabolism Hypertension / complications, metabolism* Male Rats Rats, Inbred SHR Rats, Inbred WKY Risk Assessment Sphingomyelins / metabolism* Stroke / etiology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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NHLBI-HL18575/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sphingomyelins; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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