| Increased membrane permeability of apoptotic thymocytes: a flow cytometric study. | |
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MedLine Citation:
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PMID: 8404365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have recently developed a method for the separation and quantification of viable apoptotic cells without the need for permeabilisation or fixation of the cells. The method is based on the observation that apoptotic rat thymocytes fluoresce more brightly than normal cells after a brief incubation with the DNA binding dye, Hoechst 33342. In order to understand these differences, we have investigated the uptake of Hoechst 33342 by normal and apoptotic thymocytes. By staining with fluorescein diacetate, we have shown that the efflux of fluorescein from apoptotic cells is more rapid than that from normal thymocytes. This result demonstrated an increase in the permeability of the plasma membrane of the apoptotic thymocytes and it is this change which probably results in the more rapid uptake of Hoechst 33342. The data also revealed two populations of apoptotic thymocytes. |
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Authors:
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M G Ormerod; X M Sun; R T Snowden; R Davies; H Fearnhead; G M Cohen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytometry Volume: 14 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1993 |
Date Detail:
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Created Date: 1993-10-29 Completed Date: 1993-10-29 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 595-602 Citation Subset: IM |
Affiliation:
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MRC Toxicology Unit, Carshalton, Surrey, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / physiology* Benzimidazoles / pharmacokinetics Cell Membrane Permeability / physiology* Cell Nucleus / chemistry, ultrastructure Cell Separation Cells, Cultured DNA / analysis, chemistry Flow Cytometry / methods Fluoresceins Male Pyrimidinones / pharmacokinetics Rats Rats, Inbred F344 Thymus Gland / chemistry, cytology*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Benzimidazoles; 0/Fluoresceins; 0/Pyrimidinones; 23491-52-3/HOE 33342; 58823-12-4/merocyanine dye; 596-09-8/3',6'-diacetylfluorescein; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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