Document Detail


Increased mandibular condylar growth in mice with estrogen receptor beta deficiency.
MedLine Citation:
PMID:  23197372     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via estrogen receptor beta (ERβ) inhibits axial skeletal growth in female mice. However, the role of ERβ in the mandibular condyle is largely unknown. We hypothesize that female ERβ-deficient mice will have increased mandibular condylar growth compared to wild-type (WT) female mice. This study examined female 7-day-old, 49-day-old, and 120-day-old WT and ERβ knockout (KO) mice. There was a significant increase in mandibular condylar cartilage thickness as a result of an increased number of cells, in the 49-day-old and 120-day-old female ERβ KO compared with WT controls. Analysis in 49-day-old female ERβ KO mice revealed a significant increase in collagen type X, parathyroid hormone-related protein (Pthrp), and osteoprotegerin gene expression and a significant decrease in receptor activator for nuclear factor κ B ligand (Rankl) and Indian hedgehog (Ihh) gene expression, compared with WT controls. Subchondral bone analysis revealed a significant increase in total condylar volume and a decrease in the number of osteoclasts in the 49-day-old ERβ KO compared with WT female mice. There was no difference in cell proliferation in condylar cartilage between the genotypes. However, there were differences in the expression of proteins that regulate the cell cycle; we found a decrease in the expression of Tieg1 and p57 in the mandibular condylar cartilage from ERβ KO mice compared with WT mice. Taken together, our results suggest that ERβ deficiency increases condylar growth in female mice by inhibiting the turnover of fibrocartilage.
Authors:
Yosuke Kamiya; Jing Chen; Manshan Xu; Achint Utreja; Thomas Choi; Hicham Drissi; Sunil Wadhwa
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  28     ISSN:  1523-4681     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-18     Completed Date:  2013-10-18     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1127-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 American Society for Bone and Mineral Research.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Estrogen Receptor beta / genetics*
Female
Gene Expression
Insulin-Like Growth Factor I / metabolism
Male
Mandibular Condyle / growth & development*
Mice
Mice, Inbred C57BL
Mice, Knockout
Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
NIH 1R01DE020097/DE/NIDCR NIH HHS; R01 DE020097/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Receptor beta; 67763-96-6/Insulin-Like Growth Factor I
Comments/Corrections

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