| Increased lymphatic flux of hyaluronan from cat intestine during fat absorption. | |
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MedLine Citation:
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PMID: 1636716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During fat absorption, chylomicrons with sizes up to 5,000-10,000 A must traverse an interstitium that has estimated pore sizes of 120-200 A to reach the lacteals. The present experiments were performed to study the behavior of the interstitial matrix component hyaluronan during fat absorption from the intestine. Ileal segments were isolated and autoperfused in pentobarbital-anesthetized cats. A postnodal lymphatic was cannulated, and lymph flow, protein, and hyaluronan concentration in lymph were determined. In group 1, a mixture of oleic acid and taurocholate was infused into the ileal lumen, while in group 2 the animals were fed cream overnight. In group 1, control lymph flow and hyaluronan concentration averaged 53.3 +/- 16.0 (SD) microliters.min-1.100 g intestine-1 and 21.4 +/- 16.0 micrograms/ml, respectively. Administration of taurocholate and oleic acid increased lymph flow and lymph hyaluronan concentration by 100 and 50%, respectively, resulting in a nearly three-fold increase in hyaluronan flux. Subsequent increases in venous pressure increased lymph flow and reduced hyaluronan concentration in lymph to less than 3 micrograms/ml. Hyaluronan flux remained approximately 2 micrograms.min-1.100 g intestine-1 independent of lymph flow. In group 2, no lymph sample was available before administration of fat. Hyaluronan concentration at control venous pressure was 19.3 +/- 6.7 micrograms/ml and fell to 10 micrograms/ml at the highest lymph flow. Hyaluronan flux was approximately 10 micrograms.min-1.100 g intestine-1 at the highest lymph flow and venous pressure (P less than 0.05 compared with the same lymph flow in group 1).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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R K Reed; M I Townsley; V H Pitts; T C Laurent; A E Taylor |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 263 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-08-21 Completed Date: 1992-08-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G6-11 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of South Alabama, Mobile 36688. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Cats Fats / pharmacokinetics* Hyaluronic Acid / metabolism* Intestines / metabolism* Lymphatic System / metabolism* Milk Oleic Acid Oleic Acids / metabolism Osmolar Concentration Proteins / metabolism Taurocholic Acid / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL-39045/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fats; 0/Oleic Acids; 0/Proteins; 112-80-1/Oleic Acid; 81-24-3/Taurocholic Acid; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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