Document Detail

Increased lymphangiogenesis and hemangiogenesis in infant cornea.
MedLine Citation:
PMID:  21688980     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Corneal lymphangiogenesis (LG) and hemangiogenesis (HG) accompany many diseases after inflammatory, infectious, traumatic or chemical insults. They also contribute to transplant rejection. It is known that corneal transplants in infants or children have a higher rejection rate than in adults. However, it has never been studied whether infant corneas differ from adult corneas in inflammatory LG, HG, or both, which is the focus of this study.
METHODS AND RESULTS: Corneal inflammatory LG and HG were induced by a standard suture placement model in C57BL/6 mice of 3 weeks and 8 weeks of age, respectively. Corneal LG, HG, and macrophage infiltration were assessed by immunofluorescent microscopic studies using specific antibodies against CD31 (a panendothelial cell marker), LYVE-1 (a lymphatic marker), and F4/80 (a macrophage marker). Blood vessels were also examined by ophthalmic slit-lamp microscopic assays in vivo. Digital images were analyzed by NIH Image J software. It was found, for the first time, that infant corneas exhibited a higher level of LG, HG, and macrophage infiltration during inflammation. Infant lymphatic and blood vessels demonstrated greater density and invasion area but similar branching points. Additionally, infant lymphatic vessels were also of larger diameter.
CONCLUSIONS: Infant and adult corneas differ greatly in their inflammatory responses of LG, HG, and macrophage infiltration. These novel findings will shed some light on our understanding of the LG and HG processes, as well as the development of new therapeutic protocols for corneal diseases, particularly, in infants or children, where an early restoration of sight is critically important in preventing amblyopia or permanent vision loss.
Don Yuen; Rose Leu; Anna Sadovnikova; Lu Chen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lymphatic research and biology     Volume:  9     ISSN:  1557-8585     ISO Abbreviation:  Lymphat Res Biol     Publication Date:  2011  
Date Detail:
Created Date:  2011-06-21     Completed Date:  2011-10-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101163587     Medline TA:  Lymphat Res Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  109-14     Citation Subset:  IM    
Center for Eye Disease and Development, Program in Vision Science, School of Optometry, University of California , Berkeley, USA.
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MeSH Terms
Cornea / blood supply*
Mice, Inbred C57BL
Microscopy, Fluorescence
Retinal Vessels / growth & development*

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