Document Detail


Increased luminal mucin does not disturb glucose or ovalbumin absorption in rats fed insoluble dietary fiber.
MedLine Citation:
PMID:  16988114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We tested whether increased mucin secretion due to ingestion of insoluble dietary fiber (IDF) affects small intestinal nutrient absorption in rats. Polystyrene foam (PSF) with a true expansion ratio of 54.9 was used as a model for IDF with high bulk-forming properties. In Expt. 1, rats were fed a control diet or diet containing 50 g PSF/kg for 1, 3, 5, or 7 d. Small intestinal mucin fractions were isolated, and O-linked oligosaccharide chains were measured. The luminal mucin content reached a maximum within 5 d after PSF ingestion. In Expt. 2, rats were fed a control diet or diet containing 50 g PSF/kg for 7 d, and then all rats were switched to the control diet for 1, 3, or 5 d. The increased capacity for luminal mucin secretion disappeared within 5 d after ceasing PSF ingestion. In Expt. 3, rats were fed a control diet or diet containing 70 g PSF/kg for 7 d. Glucose (1g/kg) was administered orally after 12 h of food deprivation. The blood glucose concentrations did not differ between the groups. In Expt. 4, rats were fed a control diet or diet containing 90 g PSF/kg for 14 d. At d 7, portal cannulae were installed. A mixed solution of glucose (1g/kg) and ovalbumin (OVA, 250 mg/kg) was orally administered after 12 h of food deprivation, and responses of portal glucose and OVA concentrations were monitored for 120 min. Although luminal mucin contents were almost doubled in the 9% PSF group compared with the control group, neither portal glucose nor OVA concentration differed at any time point. The results suggest that the short-term ingestion of IDF significantly increases the luminal mucin content, but that this does not disturb nutrient absorption.
Authors:
Tatsuya Morita; Hiroki Tanabe; Hiroyuki Ito; Shunsuke Yuto; Takeshi Matsubara; Tsukasa Matsuda; Kimio Sugiyama; Shuhachi Kiriyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  136     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-21     Completed Date:  2006-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2486-91     Citation Subset:  IM    
Affiliation:
Faculty of Nutritional Sciences, University of Shizuoka, Yada 52-1, Shizuoka 422-8526, Japan. actmori@agr.shizuoka.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Conformation
Dietary Fiber / administration & dosage*
Electrophoresis, Polyacrylamide Gel
Food Deprivation
Glucose / administration & dosage,  pharmacokinetics*
Glucose Tolerance Test
Intestinal Absorption / physiology*
Intestine, Small / metabolism
Male
Mucins / analysis,  chemistry,  secretion*
Oligosaccharides / analysis,  chemistry
Ovalbumin / administration & dosage,  pharmacokinetics*
Polystyrenes
Rats
Rats, Sprague-Dawley
Rats, Wistar
Solubility
Chemical
Reg. No./Substance:
0/Mucins; 0/Oligosaccharides; 0/Polystyrenes; 50-99-7/Glucose; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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