Document Detail


Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings.
MedLine Citation:
PMID:  12376419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thermogenic endurance and development of metabolic cold adaptation in birds may critically depend on their ability to synthesize and use fatty acids (FA) as fuel substrates. Hepatic lipogenesis and the capacity to oxidize FA in thermogenic tissues were measured in cold-acclimated (CA) ducklings (Cairina moschata) showing original mechanisms of metabolic cold adaptation in the absence of brown adipose tissue, the specialized thermogenic tissue of rodents. The rate of FA synthesis from [U-(14)C]glucose and from [1-(14)C]acetate, measured in incubated hepatocytes isolated from 5-wk-old thermoneutral (TN; 25 degrees C) or CA (4 degrees C) fed ducklings, was higher than in other species. Hepatic de novo lipogenesis was further increased by cold acclimation with both glucose (+194%) and acetate (+111%) as precursor. Insulin slightly increased (+11-14%) hepatic lipogenesis from both precursors in CA ducklings, whereas glucagon was clearly inhibitory (-29 to -51%). Enhanced de novo lipogenesis was associated with higher (+171%) hepatocyte activity of glucose oxidation and larger capacity (+50 to +100%) of key lipogenic enzymes. The potential for FA oxidation was higher in liver (+61%) and skeletal muscle (+29 to +81%) homogenates from CA than from TN ducklings, suggesting that the higher hepatic lipogenesis may fuel oxidation in thermogenic tissues. Present data underline the high capacity to synthesize lipids from glucose in species like muscovy ducks susceptible to hepatic steatosis. Lipogenic capacity can be further increased in the cold and may represent an important step in the metabolic adaptation to cold of growing ducklings.
Authors:
E Bedu; F Chainier; B Sibille; R Meister; G Dallevet; D Garin; C Duchamp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  283     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-11     Completed Date:  2002-11-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1245-53     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, Centre National de la Recherche Scientifique-Université Claude Bernard Lyon 1, Unité Mixte de Recherches 5123, Villeurbanne, France. elodie.bedu@univ-lyon1.fr
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MeSH Terms
Descriptor/Qualifier:
Acclimatization / physiology*
Acetyl-CoA Carboxylase / metabolism
Animals
Body Temperature Regulation / physiology
Cell Separation
Cold Temperature*
Ducks / physiology*
Fatty Acid Synthetase Complex / metabolism
Fatty Acids / metabolism
Glucagon / physiology
Glucose / metabolism
Glucosephosphate Dehydrogenase / metabolism
Hepatocytes / metabolism*
Insulin / physiology
Kinetics
Lipids / biosynthesis*
Liver / metabolism,  physiology
Male
Muscle, Skeletal / enzymology,  metabolism
Oleic Acid / metabolism
Oxidation-Reduction
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipids; 11061-68-0/Insulin; 112-80-1/Oleic Acid; 50-99-7/Glucose; 9007-92-5/Glucagon; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 6.-/Fatty Acid Synthetase Complex; EC 6.4.1.2/Acetyl-CoA Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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