| Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings. | |
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MedLine Citation:
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PMID: 12376419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thermogenic endurance and development of metabolic cold adaptation in birds may critically depend on their ability to synthesize and use fatty acids (FA) as fuel substrates. Hepatic lipogenesis and the capacity to oxidize FA in thermogenic tissues were measured in cold-acclimated (CA) ducklings (Cairina moschata) showing original mechanisms of metabolic cold adaptation in the absence of brown adipose tissue, the specialized thermogenic tissue of rodents. The rate of FA synthesis from [U-(14)C]glucose and from [1-(14)C]acetate, measured in incubated hepatocytes isolated from 5-wk-old thermoneutral (TN; 25 degrees C) or CA (4 degrees C) fed ducklings, was higher than in other species. Hepatic de novo lipogenesis was further increased by cold acclimation with both glucose (+194%) and acetate (+111%) as precursor. Insulin slightly increased (+11-14%) hepatic lipogenesis from both precursors in CA ducklings, whereas glucagon was clearly inhibitory (-29 to -51%). Enhanced de novo lipogenesis was associated with higher (+171%) hepatocyte activity of glucose oxidation and larger capacity (+50 to +100%) of key lipogenic enzymes. The potential for FA oxidation was higher in liver (+61%) and skeletal muscle (+29 to +81%) homogenates from CA than from TN ducklings, suggesting that the higher hepatic lipogenesis may fuel oxidation in thermogenic tissues. Present data underline the high capacity to synthesize lipids from glucose in species like muscovy ducks susceptible to hepatic steatosis. Lipogenic capacity can be further increased in the cold and may represent an important step in the metabolic adaptation to cold of growing ducklings. |
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Authors:
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E Bedu; F Chainier; B Sibille; R Meister; G Dallevet; D Garin; C Duchamp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 283 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-11 Completed Date: 2002-11-06 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1245-53 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, Centre National de la Recherche Scientifique-Université Claude Bernard Lyon 1, Unité Mixte de Recherches 5123, Villeurbanne, France. elodie.bedu@univ-lyon1.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acclimatization
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physiology* Acetyl-CoA Carboxylase / metabolism Animals Body Temperature Regulation / physiology Cell Separation Cold Temperature* Ducks / physiology* Fatty Acid Synthetase Complex / metabolism Fatty Acids / metabolism Glucagon / physiology Glucose / metabolism Glucosephosphate Dehydrogenase / metabolism Hepatocytes / metabolism* Insulin / physiology Kinetics Lipids / biosynthesis* Liver / metabolism, physiology Male Muscle, Skeletal / enzymology, metabolism Oleic Acid / metabolism Oxidation-Reduction |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipids; 11061-68-0/Insulin; 112-80-1/Oleic Acid; 50-99-7/Glucose; 9007-92-5/Glucagon; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 6.-/Fatty Acid Synthetase Complex; EC 6.4.1.2/Acetyl-CoA Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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