Document Detail

Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings.
MedLine Citation:
PMID:  12376419     Owner:  NLM     Status:  MEDLINE    
Thermogenic endurance and development of metabolic cold adaptation in birds may critically depend on their ability to synthesize and use fatty acids (FA) as fuel substrates. Hepatic lipogenesis and the capacity to oxidize FA in thermogenic tissues were measured in cold-acclimated (CA) ducklings (Cairina moschata) showing original mechanisms of metabolic cold adaptation in the absence of brown adipose tissue, the specialized thermogenic tissue of rodents. The rate of FA synthesis from [U-(14)C]glucose and from [1-(14)C]acetate, measured in incubated hepatocytes isolated from 5-wk-old thermoneutral (TN; 25 degrees C) or CA (4 degrees C) fed ducklings, was higher than in other species. Hepatic de novo lipogenesis was further increased by cold acclimation with both glucose (+194%) and acetate (+111%) as precursor. Insulin slightly increased (+11-14%) hepatic lipogenesis from both precursors in CA ducklings, whereas glucagon was clearly inhibitory (-29 to -51%). Enhanced de novo lipogenesis was associated with higher (+171%) hepatocyte activity of glucose oxidation and larger capacity (+50 to +100%) of key lipogenic enzymes. The potential for FA oxidation was higher in liver (+61%) and skeletal muscle (+29 to +81%) homogenates from CA than from TN ducklings, suggesting that the higher hepatic lipogenesis may fuel oxidation in thermogenic tissues. Present data underline the high capacity to synthesize lipids from glucose in species like muscovy ducks susceptible to hepatic steatosis. Lipogenic capacity can be further increased in the cold and may represent an important step in the metabolic adaptation to cold of growing ducklings.
E Bedu; F Chainier; B Sibille; R Meister; G Dallevet; D Garin; C Duchamp
Related Documents :
17883839 - In vitro metabolism of beclomethasone dipropionate, budesonide, ciclesonide, and flutic...
11465339 - Technical aspects of microdialysis of human breast.
7591199 - Membrane fatty acids of breast carcinoma: contribution of host fatty acids and tumor pr...
20622189 - Correlations between fat depot traits and fatty acid composition in abdominal subcutane...
8685239 - Fatty acid composition of adipose tissue in humans. implications for the dietary fat-se...
16460979 - Lipid content and fatty acid distribution in tissues from portuguese dogfish, leafscale...
25481749 - Molecular cloning and characterization of a nematode polyprotein antigen/allergen from ...
11860139 - Chiral separation of underivatized amino acids by ligand-exchange capillary electrophor...
20674289 - Nanomicelle with long-term circulation and enhanced stability of camptothecin based on ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  283     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-11     Completed Date:  2002-11-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1245-53     Citation Subset:  IM    
Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, Centre National de la Recherche Scientifique-Université Claude Bernard Lyon 1, Unité Mixte de Recherches 5123, Villeurbanne, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acclimatization / physiology*
Acetyl-CoA Carboxylase / metabolism
Body Temperature Regulation / physiology
Cell Separation
Cold Temperature*
Ducks / physiology*
Fatty Acid Synthetase Complex / metabolism
Fatty Acids / metabolism
Glucagon / physiology
Glucose / metabolism
Glucosephosphate Dehydrogenase / metabolism
Hepatocytes / metabolism*
Insulin / physiology
Lipids / biosynthesis*
Liver / metabolism,  physiology
Muscle, Skeletal / enzymology,  metabolism
Oleic Acid / metabolism
Reg. No./Substance:
0/Fatty Acids; 0/Lipids; 11061-68-0/Insulin; 112-80-1/Oleic Acid; 50-99-7/Glucose; 9007-92-5/Glucagon; EC Dehydrogenase; EC 6.-/Fatty Acid Synthetase Complex; EC Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Beta-adrenoceptor control of G protein function in the neonate: determinant of desensitization or se...
Next Document:  Oxygen-limited thermal tolerance in Antarctic fish investigated by MRI and (31)P-MRS.