| Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro. | |
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MedLine Citation:
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PMID: 18667023 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasma membranes are essential components of living cells, and phospholipids are major components of cellular membranes. Here, we used liquid chromatography/mass spectrometry to investigate changes in the membrane phospholipid content that occur in association with aging. Our results indicate that the levels of a particular species of phosphatidylcholine comprised of stearic acid and arachidonic acid increased with age. To determine the reason for the increased levels of this particular phosphatidylcholine, we examined the effect of highly unsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, on cellular aging. Applied arachidonic acid was incorporated into phosphatidylcholine molecules, but neither arachidonic acid nor other related unsaturated fatty acids had any effect. We conclude that increased levels of this distinctive phosphatidylcholine are a result of in vitro senescence. |
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Authors:
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Eiji Naru; Yasukazu Takanezawa; Misako Kobayashi; Yuko Misaki; Kazuhiko Kaji; Kumi Arakane |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Human cell : official journal of Human Cell Research Society Volume: 21 ISSN: 0914-7470 ISO Abbreviation: Hum. Cell Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-31 Completed Date: 2008-10-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912329 Medline TA: Hum Cell Country: Japan |
Other Details:
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Languages: eng Pagination: 70-8 Citation Subset: IM |
Affiliation:
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Research and Development Division, KOSE Corporation, Tokyo, 114-0005, Japan. e-naru@kose.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Arachidonic Acid / metabolism, pharmacology Cell Aging / drug effects* Cells, Cultured Chromatography, Liquid Dermis / cytology* Eicosapentaenoic Acid / metabolism, pharmacology Fibroblasts / metabolism* Humans Mass Spectrometry Phosphatidylcholines / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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