Document Detail

Increased levels of oxidized methionine residues in bronchoalveolar lavage fluid proteins from patients with idiopathic pulmonary fibrosis.
MedLine Citation:
PMID:  1990939     Owner:  NLM     Status:  MEDLINE    
Phagocytic cells are believed to play a crucial role in the development of inflammatory lung diseases. We assumed that the oxidation of methionine (met) to methionine sulfoxide [met(O)] by oxygen-derived free radicals released from phagocytes is one parameter to identify the oxidative mechanisms of lung injury. To test this hypothesis we determined the molar ratio of met(O)/met in the soluble protein fraction of bronchoalveolar lavage (BAL) fluids from healthy nonsmokers and from nonsmoking patients with idiopathic pulmonary fibrosis (IPF) or sarcoidosis. The met(O)/met ratio of the healthy nonsmoker group (n = 11) was 0.046 +/- 0.008 (mean +/- SEM). In contrast, the met(O)/met ratio of the nonsmoking IPF group (n = 11) was significantly increased to 0.223 +/- 0.053 (p less than 0.0002). The BAL fluids of this group showed strongly increased numbers of neutrophils but normal numbers of alveolar macrophages (AM). In the sarcoidosis group (n = 10) the met(O)/met ratio (0.048 +/- 0.010) was not significantly different from control values. A close relationship was found between the met(O)/met ratios and the relative as well as the absolute neutrophil counts (r = 0.86; p less than 0.0002; n = 22). In contrast, no significant correlation was found between the met(O)/met ratios and the absolute AM counts (r = 0.22; p = 0.32; n = 22). We conclude that mechanisms of oxidative lung injury in IPF can be characterized by oxidation of met and that this oxidation may be mediated by neutrophils.
K Maier; L Leuschel; U Costabel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  143     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-03-07     Completed Date:  1991-03-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-4     Citation Subset:  AIM; IM    
Projekt Inhalation der Gesellschaft für Strahlen- und Umweltforschung München, Neuherberg, FRG.
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MeSH Terms
Aging / physiology
Bronchoalveolar Lavage Fluid / cytology,  metabolism*
Drug Residues
Leukocyte Count
Lymphocytes / pathology
Methionine / analogs & derivatives,  metabolism*
Middle Aged
Neutrophils / pathology
Proteins / metabolism*
Pulmonary Fibrosis / metabolism*,  pathology
Reference Values
Sarcoidosis / metabolism,  pathology
Reg. No./Substance:
0/Proteins; 454-41-1/methionine sulfoxide; 63-68-3/Methionine

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