| Increased levels of osteopontin in sputum supernatant in severe refractory asthma. | |
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MedLine Citation:
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PMID: 20805171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Osteopontin (OPN) is a glycoprotein that has been associated with inflammation and fibrosis. Severe refractory asthma (SRA) is characterised by an intense inflammatory and remodelling process. The aim of this study was to investigate the levels of OPN in sputum supernatants of patients with SRA, to compare them with milder forms of the disease and to investigate their possible association with mediators and cells involved in the inflammatory and remodelling process. METHODS: 33 patients with SRA, 29 with moderate asthma, 21 with steroid-naïve asthma and 20 healthy subjects were studied. All subjects underwent lung function tests, bronchial hyper-responsiveness assessment and sputum induction for cell count identification and measurement of OPN, vascular endothelial growth factor, transforming growth factor beta1 (TGF-beta1), cysteinyl leukotrienes, interleukin 13 (IL-13), eosinophilic cationic protein (ECP) and IL-8 in sputum supernatants. RESULTS: Median (IQR) OPN levels (pg/ml) were significantly higher in patients with SRA than in those with moderate asthma, steroid-naive asthma and healthy control subjects (1840 (1125-11000) vs 130 (100-210) vs 100 (67-130) vs 50 (42-70), respectively, p<0.001). Regression analysis showed a significant association between log OPN and sputum eosinophils, cysteinyl leukotrienes, IL-13, TGF-beta1 and ECP. TGF-beta1 represented the strongest association with OPN. The above associations were not observed in milder forms of the disease or in healthy subjects. CONCLUSIONS: The results indicate that OPN levels are higher in SRA than in less severe forms of the disease. Moreover, OPN is associated with mediators involved in both the inflammatory and remodelling process such as TGF-beta1, IL-13 and cysteinyl leukotrienes only in SRA. |
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Authors:
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Vasiliki Delimpoura; Petros Bakakos; Eleni Tseliou; Vasiliki Bessa; Georgios Hillas; Davina C M Simoes; Spyros Papiris; Stelios Loukides |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Thorax Volume: 65 ISSN: 1468-3296 ISO Abbreviation: Thorax Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-31 Completed Date: 2010-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417353 Medline TA: Thorax Country: England |
Other Details:
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Languages: eng Pagination: 782-6 Citation Subset: IM |
Affiliation:
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2nd Respiratory Medicine Department, University of Athens Medical School, Attikon Hospital, Smolika 2 16673, Athens, Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Airway Remodeling / physiology Asthma / drug therapy, metabolism*, physiopathology Biological Markers / analysis Cohort Studies Female Forced Expiratory Volume / physiology Glucocorticoids / therapeutic use Humans Inflammation Mediators / analysis Male Middle Aged Osteopontin / analysis* Sputum / chemistry* Vital Capacity / physiology |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Glucocorticoids; 0/Inflammation Mediators; 106441-73-0/Osteopontin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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