Document Detail


Increased levels of erucic acid in Brassica carinata by co-suppression and antisense repression of the endogenous FAD2 gene.
MedLine Citation:
PMID:  15885619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erucic acid and its derivatives represent important industrial feedstock compounds, and there is an increasing demand for the production of high erucate oils in this regard. Our goal therefore, is to develop high erucic acid (HEA) Brassicaceae lines with increased proportions of erucic acid and very long-chain fatty acids (VLCFAs). We proposed that oleate availability may be a rate-limiting factor in the biosynthesis of erucic acid. We have tried to address this question by manipulating the expression of the endogenous FAD2 gene in B. carinata using co-supression and antisense approaches. Both methods resulted in transgenic lines exhibiting decreased proportions of polyunsaturated C18 fatty acids (18:2+18:3) and concomitant and significantly increased proportions of 18:1, 22:1 and total VLCFAs. Co-suppressed FAD2 B. carinata lines exhibited 3-18% decreases in 18:2, 22-49% decreases in 18:3 and significantly increased proportions of 18:1 (36-99%), 22:1 (12-27%) and VLCFAs (6-15%). Transgenic B. carinata lines developed using an antisense FAD2 approach exhibited decreased proportions of 18:2 and 18:3 (9-39% and 33-48%, respectively) and significantly increased proportions of 18:1 (54-130%), 22:1 (5-19%) and VLCFAs (6-21%). The possibility of using these approaches to produce prototype transgenic germplasm of the Brassicaceae accumulating seed oils with improved proportions of erucic and other VLCFAs is discussed.
Authors:
Ashok Jadhav; Vesna Katavic; Elizabeth-France Marillia; E Michael Giblin; Dennis L Barton; Arvind Kumar; Cory Sonntag; Vivijan Babic; Wilfred A Keller; David C Taylor
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metabolic engineering     Volume:  7     ISSN:  1096-7176     ISO Abbreviation:  Metab. Eng.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-11     Completed Date:  2005-10-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-20     Citation Subset:  IM    
Affiliation:
National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9.
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MeSH Terms
Descriptor/Qualifier:
Brassica / genetics*,  metabolism*
Erucic Acids / metabolism*
Fatty Acid Desaturases / deficiency*,  genetics*
Fatty Acids / metabolism*
Gene Silencing / physiology
Genetic Enhancement / methods*
Protein Engineering / methods*
RNA, Antisense / genetics
Suppression, Genetic / genetics
Chemical
Reg. No./Substance:
0/Erucic Acids; 0/Fatty Acids; 0/RNA, Antisense; 1072-39-5/erucic acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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