Document Detail

Increased levels of N(epsilon)-(carboxymethyl)lysine in epithelial lining fluid from peripheral airways in patients with chronic obstructive pulmonary disease: a pilot study.
MedLine Citation:
PMID:  20236091     Owner:  NLM     Status:  MEDLINE    
It has been reported that small airway inflammation is closely associated with the severity of airflow limitation in COPD (chronic obstructive pulmonary disease). We tested a new method of measurement of biochemical constituents in ELF (epithelial lining fluid) obtained separately from the central or peripheral airways using a bronchoscopic microsampling technique. The present study was designed to determine the validity of measuring CML [N(epsilon)-(carboxymethyl)lysine] levels in ELF for the assessment of small airway inflammation in COPD. Ten non-smokers, ten current smokers and 16 COPD patients were included in the present study. Concentrations of CML, 8-isoprostane and IL-8 (interleukin-8) were measured in ELF separately from the central or peripheral airways. CML levels in central airways did not differ significantly, but were markedly higher in peripheral than in central airways in the three groups. However, CML levels in peripheral airways of COPD patients were significantly higher than those in non-smokers and current smokers. In COPD patients, the CML level in peripheral airways was significantly correlated with FEV1 (forced expiratory volume in 1 s) (r=-0.82, P=0.002) and FEV1/FVC (forced vital capacity) (r=-0.57, P=0.03). Moreover, CML levels in peripheral airways were significantly correlated with levels of both 8-isoprostane (r=0.76, P=0.003) and IL-8 (r=0.67, P=0.01). In conclusion, these findings suggest that elevated levels of CML in ELF from peripheral airways were observed in COPD patients, and this parameter was correlated with the severity of airflow limitation.
Hiroshi Kanazawa; Toyoki Kodama; Kazuhisa Asai; Saeko Matsumura; Kazuto Hirata
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-06
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  119     ISSN:  1470-8736     ISO Abbreviation:  Clin. Sci.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-06     Completed Date:  2010-09-01     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  143-9     Citation Subset:  IM    
Department of Respiratory Medicine, Graduate School of Medicine, Osaka City University, Asahi-machi, Abenoku Osaka, Japan.
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MeSH Terms
Bronchioles / chemistry
Dinoprost / analogs & derivatives,  analysis
Forced Expiratory Volume
Interleukin-8 / analysis
Lysine / analogs & derivatives*,  analysis
Middle Aged
Pilot Projects
Pulmonary Disease, Chronic Obstructive / metabolism*,  physiopathology
Respiratory Mucosa / chemistry
Smoking / metabolism,  physiopathology
Specimen Handling / methods
Vital Capacity
Reg. No./Substance:
0/Interleukin-8; 27415-26-5/8-epi-prostaglandin F2alpha; 551-11-1/Dinoprost; 56-87-1/Lysine; 5746-04-3/N(6)-carboxymethyllysine

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