Document Detail

Increased levels of the 14-3-3 η and γ proteins in the synovial fluid of dogs with unilateral cranial cruciate ligament rupture.
MedLine Citation:
PMID:  22468024     Owner:  NLM     Status:  MEDLINE    
The present study investigated whether the 14-3-3 η and γ proteins, which are potent matrix metalloprotease (MMP) stimulators, are detectable in the synovial fluid of dogs with cranial cruciate ligament rupture (CCLR). Synovial fluid samples from 7 dogs with unilateral CCLR and control samples from 4 dogs without a history of any joint inflammation or any other abnormalities underwent Western blot analysis for the 14-3-3 η, γ, and σ proteins as well as MMP-1 and MMP-3. Craniocaudal and lateral radiographic projections of the stifle joint were evaluated for the presence and severity of 13 specific radiographic markers of osteoarthritis and graded numerically. The Spearman method was used to detect any correlation between the 14-3-3-η level in the synovial fluid and the radiograph-based grade. The η isoform was present only in the samples from the dogs with CCLR. The levels of 14-3-3-γ, MMP-1, and MMP-3 were significantly higher in the samples from the dogs with CCLR than in the control samples (P < 0.05). However, there was no significant difference between the CCLR and control samples in the level of the σ isoform. The Spearman method showed a significant correlation between the 14-3-3-η level in the synovial fluid and the presence of either patellar osteophytes or lateral or medial (or both) condylar periarticular osteophytes (P < 0.05). The MMP stimulatory effect of the 14-3-3 η and γ isoforms may be the reason for the high levels of MMP-1 and MMP-3 observed. Thus, 14-3-3 proteins, especially the η isoform, may be important markers of osteoarthritis caused by CCLR.
Kamran Sardari; Claudia Chavez-Muñoz; Ruhangiz T Kilani; Terri Schiller; Aziz Ghahary
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire     Volume:  75     ISSN:  0830-9000     ISO Abbreviation:  Can. J. Vet. Res.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2012-04-02     Completed Date:  2012-07-26     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8607793     Medline TA:  Can J Vet Res     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  271-7     Citation Subset:  IM    
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
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MeSH Terms
14-3-3 Proteins / analysis,  genetics,  metabolism*
Anterior Cruciate Ligament / injuries*
Dogs / injuries*
Hindlimb / injuries
Protein Isoforms
Rupture / metabolism,  veterinary
Synovial Fluid / chemistry*
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/14-3-3 Proteins; 0/Protein Isoforms

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