Document Detail


Increased isoprostane levels in oleic acid-induced lung injury.
MedLine Citation:
PMID:  19664592     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2alpha). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.
Authors:
Koichi Ono; Tomonobu Koizumi; Kenji Tsushima; Sumiko Yoshikawa; Toshiki Yokoyama; Rikimaru Nakagawa; Toru Obata
Publication Detail:
Type:  Journal Article     Date:  2009-08-05
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  388     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  297-300     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology and Resuscitation, Shinshu University School of Medicine, Matsumoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acute Lung Injury / blood,  chemically induced,  metabolism*
Animals
Disease Models, Animal
Isoprostanes / analysis,  blood,  metabolism*
Oleic Acid / toxicity*
Sheep
Chemical
Reg. No./Substance:
0/Isoprostanes; 112-80-1/Oleic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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