Document Detail

Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism.
MedLine Citation:
PMID:  21798334     Owner:  NLM     Status:  MEDLINE    
We have previously shown that copper supplementation extends the replicative life span of Saccharomyces cerevisiae when grown under conditions forcing cells to respire. We now show that copper's effect on life span is through Fet3p, a copper containing enzyme responsible for high affinity transport of iron into yeast cells. Life span extensions can also be obtained by supplementing the growth medium with 1mM ferric chloride. Extension by high iron levels is still dependent on the presence of Fet3p. Life span extension by iron or copper requires growth on media containing glycerol as the sole carbon source, which forces yeast to respire. Yeast grown on glucose containing media supplemented with iron show no extension of life span. The iron associated with cells grown in media supplemented with copper or iron is 1.4-1.8 times that of cells grown without copper or iron supplementation. As with copper supplementation, iron supplementation partially rescues the life span of superoxide dismutase mutants. Cells grown with copper supplementation display decreased production of superoxide as measured by dihydroethidium staining.
Gabriela Botta; Christina S Turn; Nicholas J Quintyne; Paul A Kirchman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-07-26
Journal Detail:
Title:  Experimental gerontology     Volume:  46     ISSN:  1873-6815     ISO Abbreviation:  Exp. Gerontol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-05     Completed Date:  2011-11-15     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  0047061     Medline TA:  Exp Gerontol     Country:  England    
Other Details:
Languages:  eng     Pagination:  827-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Biological Transport / genetics
Cation Transport Proteins / genetics*,  metabolism
Cell Respiration / genetics*,  physiology
Copper / metabolism*,  pharmacology
Culture Media
Gene Expression Regulation, Fungal / genetics*
Glycerol / pharmacology
Iron / metabolism,  pharmacology*
Life Expectancy
Mitochondria / genetics,  metabolism*
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae Proteins / genetics
Grant Support
1 R15 AG021956-01/AG/NIA NIH HHS; R15 AG021956/AG/NIA NIH HHS; R15 AG021956-01/AG/NIA NIH HHS
Reg. No./Substance:
0/Cation Transport Proteins; 0/Culture Media; 0/Saccharomyces cerevisiae Proteins; 789U1901C5/Copper; E1UOL152H7/Iron; PDC6A3C0OX/Glycerol

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