| Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism. | |
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MedLine Citation:
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PMID: 21798334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that copper supplementation extends the replicative life span of Saccharomyces cerevisiae when grown under conditions forcing cells to respire. We now show that copper's effect on life span is through Fet3p, a copper containing enzyme responsible for high affinity transport of iron into yeast cells. Life span extensions can also be obtained by supplementing the growth medium with 1mM ferric chloride. Extension by high iron levels is still dependent on the presence of Fet3p. Life span extension by iron or copper requires growth on media containing glycerol as the sole carbon source, which forces yeast to respire. Yeast grown on glucose containing media supplemented with iron show no extension of life span. The iron associated with cells grown in media supplemented with copper or iron is 1.4-1.8 times that of cells grown without copper or iron supplementation. As with copper supplementation, iron supplementation partially rescues the life span of superoxide dismutase mutants. Cells grown with copper supplementation display decreased production of superoxide as measured by dihydroethidium staining. |
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Authors:
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Gabriela Botta; Christina S Turn; Nicholas J Quintyne; Paul A Kirchman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-07-26 |
Journal Detail:
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Title: Experimental gerontology Volume: 46 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-05 Completed Date: 2011-11-15 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 827-32 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431, United States. Gabriela.Botta@biomet.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport / genetics Cation Transport Proteins / genetics*, metabolism Cell Respiration / genetics*, physiology Copper / metabolism*, pharmacology Culture Media Gene Expression Regulation, Fungal / genetics* Glycerol / pharmacology Iron / metabolism, pharmacology* Life Expectancy Mitochondria / genetics, metabolism* Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
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1 R15 AG021956-01/AG/NIA NIH HHS; R15 AG021956-01/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/Culture Media; 0/Saccharomyces cerevisiae Proteins; 56-81-5/Glycerol; 7439-89-6/Iron; 7440-50-8/Copper |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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