Document Detail

Increased intramuscular nerve branching and inhibition of programmed cell death of chick embryo motoneurons by immunoglobulins from patients with motoneuron disease.
MedLine Citation:
PMID:  20800908     Owner:  NLM     Status:  MEDLINE    
Massive programmed cell death (PCD) of developing chick embryo motoneurons (MNs) occurs in a well defined temporal and spatial sequence between embryonic day (E) 6 and E10. We have found that, when administered in ovo, either circulating immunoglobulins G (IgGs) or cerebrospinal fluid from patients with MN disease can rescue a significant number of chick embryo MNs from normally occurring PCD. An increase of branching of intramuscular nerves was also observed that may account for the rescuing effects of pathologic IgGs. Proteomic analysis and further analysis by ELISA indicated that these effects may be mediated by the interaction of circulating human immunoglobulins with proteins of the semaphorin family.
Sara Hernández; Laura Texidó; Jordi Calderó; Dolors Ciutat; Lídia Piedrafita; Anna Casanovas; Joan Blasi; Carles Solsona; Mònica Povedano; Ricardo Rojas; Isabel Illa; James Caress; David Prevette; Ronald W Oppenheim; Carol Milligan; Josep E Esquerda
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  229     ISSN:  1872-8421     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  157-68     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Universitat de Lleida, Facultat de Medicina, Departament de Medicina Experimental, Lleida, Catalonia, Spain.
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MeSH Terms
Analysis of Variance
Apoptosis / drug effects*
Cell Survival / drug effects
Cells, Cultured
Cercopithecus aethiops
Chick Embryo
Dose-Response Relationship, Drug
Electrophoresis, Gel, Two-Dimensional / methods
Enzyme-Linked Immunosorbent Assay / methods
Ganglia, Spinal / cytology
Growth Cones / drug effects
Immunoglobulins / immunology,  pharmacology*
Motor Neuron Disease / blood,  immunology*
Motor Neurons / cytology,  drug effects*
Muscle, Skeletal / embryology,  innervation*
Neuromuscular Junction / drug effects*,  physiology
Proteomics / methods
Semaphorins / metabolism
Serum / chemistry,  immunology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Statistics as Topic
Statistics, Nonparametric
Transfection / methods
Tubulin / metabolism
Reg. No./Substance:
0/Immunoglobulins; 0/Semaphorins; 0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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