Document Detail


Increased intestinal inflammatory response and gut barrier dysfunction in Nrf2-deficient mice after traumatic brain injury.
MedLine Citation:
PMID:  18722136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To explore the role of nuclear factor erythroid 2-related factor 2 (Nrf2) in traumatic brain injury (TBI)-induced intestinal inflammatory response and gut barrier dysfunction in the mice. METHODS: Wild-type Nrf2 (+/+) and Nrf2 (-/-)-deficient mice were subjected to a moderately severe weight-drop impact-acceleration head injury. We measured nuclear factor kappa B (NF-kappaB) by electrophoretic mobility shift assay (EMSA); tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) by enzyme-linked immunosorbent assay (ELISA); intercellular adhesion molecule-1 (ICAM-1) by immunohistochemistry; intestinal permeability by lactulose/mannitol (L/M) test; plasma endotoxin by chromogenic limulus amebocyte lysate test. RESULTS: Intestinal levels of NF-kappaB, pro-inflammatory cytokines and ICAM-1 in Nrf2 (-/-)-deficient mice were significantly higher compared with Nrf2 (+/+) mice at 24h after TBI. Furthermore, higher intestinal permeability and plasma level of endotoxin were observed in the Nrf2 (-/-) mice compared with Nrf2 (+/+) mice. CONCLUSION: Nrf2 plays an important protective role in limiting intestinal inflammatory response and gut barrier dysfunction after TBI.
Authors:
Wei Jin; Handong Wang; Yan Ji; Qingang Hu; Wei Yan; Gang Chen; Hongxia Yin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-21
Journal Detail:
Title:  Cytokine     Volume:  44     ISSN:  1096-0023     ISO Abbreviation:  Cytokine     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-17     Completed Date:  2009-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-40     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing 210002, Jiangsu Province, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Injuries / complications*,  physiopathology*
Endotoxins / blood
Inflammation / physiopathology*
Intercellular Adhesion Molecule-1 / metabolism
Interleukin-1beta / metabolism
Interleukin-6 / metabolism
Intestinal Mucosa / physiopathology
Intestines / immunology*
Male
Mice
NF-E2-Related Factor 2 / deficiency*
NF-kappa B / metabolism
Permeability
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Interleukin-1beta; 0/Interleukin-6; 0/NF-E2-Related Factor 2; 0/NF-kappa B; 0/Nfe2l2 protein, mouse; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1

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