Document Detail


Increased incidence of hyperhaploid 24,XY spermatozoa detected by three-colour FISH in a 46,XY/47,XXY male.
MedLine Citation:
PMID:  8566948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Meiotic segregation of gonosomes from a 46,XY/47,XXY male was analysed by a three-colour fluorescence in situ hybridisation (FISH) procedure. This method allows the identification of hyperhaploid spermatozoa (with 24 chromosomes), diploid spermatozoa (with 46 chromosomes) and their meiotic origin (meiosis I or II). Alpha satellite DNA probes specific for chromosomes X, Y and 1 were observed on 27,097 sperm nuclei. The proportions of X- and Y-bearing sperm were estimated to 52.78% and 43.88%, respectively. Disomy (24,XX, 24,YY, 24,X or Y,+1) and diploidy (46,XX, 46,YY, 46,XY) frequencies were close to those obtained from control sperm, whereas the frequency of hyperhaploid 24,XY spermatozoa (2.09%) was significantly increased compared with controls (0.36%). These results support the hypothesis that a few 47,XXY germ cells would be able to complete meiosis and to produce mature spermatozoa.
Authors:
E Chevret; S Rousseaux; M Monteil; Y Usson; J Cozzi; R Pelletier; B Sèle
Related Documents :
8921068 - Analysis of chromosome constitution of human spermatozoa with normal and aberrant head ...
15572858 - Acrosome reaction in spermatozoa from the amphioxus branchiostoma belcheri (cephalochor...
11173848 - Unusual triploid males in a microchromosome-carrying clone of the amazon molly, poecili...
2375878 - Subzonal transfer of multiple sperm (mist) into early human embryos.
21837708 - Establishment of a novel malt lymphoma cell line, ma-1, from a patient with t(14;18)(q3...
10591998 - Homolog pairing and meiotic progression in coprinus cinereus.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human genetics     Volume:  97     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-03-07     Completed Date:  1996-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  171-5     Citation Subset:  IM    
Affiliation:
Reproductive Biology Unit, DyOGen Laboratory, Albert Bonniot Institute, Grenoble University Medical School, La Tronche, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Chromosomes, Human, Pair 1
DNA Probes
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Klinefelter Syndrome / genetics*
Male
Meiosis / genetics*
Mosaicism / genetics*
Sex Chromosomes
Spermatozoa*
Chemical
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A common region of loss of heterozygosity in Wilms' tumor and embryonal rhabdomyosarcoma distal to t...
Next Document:  Cockayne syndrome complementation group B associated with xeroderma pigmentosum phenotype.