Document Detail

Increased in vitro thyrotropic activity of partially sialated human chorionic gonadotropin extracted from hydatidiform moles of patients with hyperthyroidism.
MedLine Citation:
PMID:  8421106     Owner:  NLM     Status:  MEDLINE    
The intrinsic thyrotropic activity of hCG purified from normal pregnancy urine has been demonstrated in several laboratories. hCG has a specific thyrotropic potency of about 0.04-0.5 microU bovine (b) TSH/IU hCG, depending on the bioassay system. The corresponding potency for hCG derived from pathological sources, such as hydatidiform moles and choriocarcinoma tissue, or from the serum of these patients has not been studied as extensively. Since the biological activity of glycoproteins can be strongly influenced by variations in the oligosaccharide side-chain composition, we have investigated the effect of anion exchange chromatography on the thyrotropic potency of hCG derived from the hydatidiform mole and serum of three hyperthyroid patients with molar pregnancy. The activity for the fraction of total molar hCG immunoreactivity that was not retained by an anion exchange column (0.18-0.90 microM bTSH/IU hCG) was about twice that of the corresponding serum and molar hCG fraction eluting during the NaCl gradient elution (0.08-0.40 microU bTSH/IU hCG). The unretained hCG fraction corresponds to a previously described hCG precursor that is partially desialated in the C-terminal region of the beta-subunit.
A E Pekary; I M Jackson; T M Goodwin; X P Pang; M D Hein; J M Hershman
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  76     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-02-17     Completed Date:  1993-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-4     Citation Subset:  AIM; IM    
Endocrinology Research Laboratory, West Los Angeles Veterans Administration Medical Center, Wadsworth Division, California 90073.
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MeSH Terms
Biological Assay
Chorionic Gonadotropin / isolation & purification*,  pharmacology*,  urine
Chromatography, Ion Exchange
Hydatidiform Mole / blood,  chemistry*,  complications,  surgery
Hyperthyroidism / blood,  complications*
Iodides / metabolism
Recombinant Proteins / pharmacology
Sialic Acids / analysis
Thymidine / metabolism
Thyroid Neoplasms
Thyrotropin / pharmacology
Tumor Cells, Cultured
Uterine Neoplasms / blood,  chemistry*,  complications,  surgery
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Iodides; 0/Recombinant Proteins; 0/Sialic Acids; 50-89-5/Thymidine; 9002-71-5/Thyrotropin

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