| Increased in-vitro incubation time of endothelial cells on fibronectin-treated ePTFE increases cell retention in blood flow. | |
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MedLine Citation:
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PMID: 1864396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endothelial cell (EC) seeding is postulated as a mechanism of improving patency of small calibre vascular grafts. However, the majority of seeded cells are lost within hours following restoration of blood flow. We postulated that incubating EC in-vitro on a graft will improve adherence and resistance to the sheer stresses of pulsatile blood flow. Fibronectin-treated ePTFE (5 cm x 4 mm ID) seeded with Indium-111-labelled autologous canine EC (1.5 x 10(5) cells/cm2) were incubated for four different time periods; 90 min, 24 h, 72 h and 6 days. Incubated grafts were subjected to blood flow of 75 ml/min for 6 h, in a canine ex-vivo arteriovenous shunt circuit. EC retention during perfusion was studied by measuring gamma activity emitted by the grafts. Cell morphology of non-perfused control groups and perfused groups was compared using scanning electron microscopy (SEM). SEM of control grafts showed progressive EC spreading on the ePTFE surface for up to 72 h incubation. Gamma activity was significantly higher at 6 h perfusion in grafts incubated for 72 h (82 +/- 4%) and 24 h (63 +/- 6%) vs. 90 min (34 +/- 13%, p less than 0.05), and between grafts incubated for 72 h vs. 6 days (55 +/- 7%, p less than 0.05). Perfused grafts incubated for 72 h showed unaltered EC morphology on SEM, few cells remained on 90 min incubated grafts. We conclude that incubating EC on fibronectin-treated ePTFE for 72 h in-vitro after seeding improves cell retention during blood flow. |
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Authors:
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E J Prendiville; J E Coleman; A D Callow; K E Gould; S Laliberte-Verdon; K Ramberg; R J Connolly |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of vascular surgery Volume: 5 ISSN: 0950-821X ISO Abbreviation: Eur J Vasc Surg Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-09-11 Completed Date: 1991-09-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8709440 Medline TA: Eur J Vasc Surg Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 311-9 Citation Subset: IM |
Affiliation:
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Department of Surgery, Tufts University School of Medicine, New England Medical Center Hospitals, Boston, Massachusetts. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Vessel Prosthesis* Cell Adhesion / drug effects, physiology Dogs Endothelium, Vascular / cytology* Fibronectins / pharmacology* Jugular Veins / cytology* Models, Cardiovascular* Polytetrafluoroethylene* Regional Blood Flow Surface Properties Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL36898-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 9002-84-0/Polytetrafluoroethylene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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