Document Detail

Increased human T lymphotropic virus type-1 (HTLV-1) proviral DNA in peripheral blood mononuclear cells and bronchoalveolar lavage cells from Japanese patients with HTLV-1-associated myelopathy.
MedLine Citation:
PMID:  8447519     Owner:  NLM     Status:  MEDLINE    
Human T lymphotropic virus type-1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is endemic in tropical areas and in southwestern Japan, and has now been identified among risk groups in the United States and some European countries. Patients with HAM/TSP may also have T lymphocyte alveolitis related to the HTLV-1 infection. To quantify proportions of HTLV-1-infected cells, a fragment of HTLV-1 proviral DNA was amplified from peripheral blood mononuclear cells (PBMC) and bronchoalveolar lavage (BAL) cells obtained from patients with HAM/TSP, non-HAM/TSP HTLV-1 carriers with chronic pulmonary inflammation, and asymptomatic HTLV-1 carriers. The proportion of HTLV-1-infected cells in PBMC from patients with HAM/TSP was much higher (3-30%) than that from asymptomatic HTLV-1 carriers (mostly < 1%), based on findings with the quantitative polymerase chain reaction. In non-HAM/TSP carriers with chronic pulmonary inflammation, HTLV-1-infected cells in PBMC were also increased, but not as markedly as that seen in patients with HAM/TSP. Integration of HTLV-1 was also noted in BAL cells from patients with HAM/TSP or non-HAM/TSP carriers with chronic pulmonary inflammation. However, in patients with HAM/TSP, there was a marked increase in HTLV-1-infected cells in the lung (7.5-30% of BAL cells), as compared with findings in non-HAM/TSP carriers (< 5%). These results suggest that increased HTLV-1 proviral DNA loading may play an important role in the development of T lymphocyte alveolitis and myelopathy in patients with HAM/TSP.
S Mita; M Sugimoto; M Nakamura; T Murakami; M Tokunaga; E Uyama; S Araki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  48     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-06     Completed Date:  1993-04-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  170-7     Citation Subset:  AIM; IM; X    
First Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
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MeSH Terms
Aged, 80 and over
Base Sequence
Bronchoalveolar Lavage Fluid / cytology,  microbiology*
Carrier State / blood,  microbiology,  pathology
Cell Count
Chronic Disease
DNA, Viral / analysis*,  blood,  chemistry
Genes, env
Human T-lymphotropic virus 1 / genetics,  isolation & purification*
Leukocytes, Mononuclear / microbiology
Middle Aged
Molecular Sequence Data
Paraparesis, Tropical Spastic / blood,  microbiology*,  pathology
Pneumonia / blood,  microbiology,  pathology
Polymerase Chain Reaction
Proviruses / genetics,  isolation & purification*
Reg. No./Substance:
0/DNA, Viral

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