| Increased function of the TRPV1 channel in small sensory neurons after local inflammation or in vitro exposure to the pro-inflammatory cytokine GRO/KC. | |
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MedLine Citation:
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PMID: 22466126 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective Inflammation at the level of the sensory dorsal root ganglia (DRGs) leads to robust mechanical pain behavior and the local inflammation has direct excitatory effects on sensory neurons including small, primarily nociceptive, neurons. These neurons express the transient receptor potential vanilloid-1 (TRPV1) channel, which integrates multiple signals of pain and inflammation. The aim of this study was to characterize the regulation of the TRPV1 channel by local DRG inflammation and by growth-related oncogene (GRO/KC, systemic name: CXCL1), a cytokine known to be upregulated in inflamed DRGs. Methods Activation of the TRPV1 receptor with capsaicin was studied with patch clamp methods in acutely isolated small-diameter rat sensory neurons in primary culture. In vivo, behavioral effects of TRPV1 and GRO/KC were examined by paw injections. Results Neurons isolated from lumbar DRGs 3 days after local inflammation showed enhanced TRPV1 function: tachyphylaxis (the decline in response to repeated applications of capsaicin) was significantly reduced. A similar effect on tachyphylaxis was observed in neurons pre-treated for 4 h in vitro with GRO/KC. This effect was blocked by H-89, a protein kinase A inhibitor. Consistent with the in vitro results, in vivo behavioral responses to paw injection of capsaicin were enhanced and prolonged by pre-injecting the paw with GRO/KC 4 h before the capsaicin injection. GRO/KC paw injections alone did not elicit pain behaviors. Conclusion Function of the TRPV1 channel is enhanced by DRG inflammation and these effects are preserved in vitro during short-term culture. The effects (decreased tachyphylaxis) are mimicked by incubation with GRO/KC, which has previously been found to be strongly upregulated in this and other pain models. |
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Authors:
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Fei Dong; Yi-Ru Du; Wenrui Xie; Judith A Strong; Xi-Jing He; Jun-Ming Zhang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience bulletin Volume: 28 ISSN: 1995-8218 ISO Abbreviation: Neurosci Bull Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101256850 Medline TA: Neurosci Bull Country: China |
Other Details:
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Languages: eng Pagination: 155-64 Citation Subset: IM |
Affiliation:
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Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0531, USA; Department of Orthopedic Surgery, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China; E-mail: Jun-Ming.Zhang@uc.edu; xijing_h@yahoo.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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