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Increased frequencies of IL-31-producing T cells are found in chronic atopic dermatitis skin.
MedLine Citation:
PMID:  22621183     Owner:  NLM     Status:  In-Data-Review    
Interleukin (IL)-31 has been associated with pruritus, a characteristic feature of atopic dermatitis (AD). Local T cell responses may be responsible for the increased level of IL-31 mRNA observed in AD. We investigated the frequency of IL-31-producing T cells in AD lesions, as well as their cytokine profile. T cells were isolated from chronic AD lesions, autologous blood and healthy donor skin. Intracellular expression of IL-31, IFN-γ, IL-13, IL-17 and IL-22 was measured using flow cytometry. T cells from AD lesions contained significantly higher percentages of IL-31-producing T cells compared to autologous blood and donor skin. Many IL-31-producing T cells co-produced IL-13 and to lesser extent IL-22, but rarely IFN-γ or IL-17. A substantial part of the IL-31-producing T cells did not co-produce any of the other cytokines and could therefore not be linked to any of the known functionally different T cell subsets. The T cell infiltrates were also relatively enriched for Th2/Tc2 and Th22/Tc22 cells, while frequencies of Th1/Tc1 and Th17 cells were decreased. This is the first report describing the detection of IL-31 at protein level in skin-infiltrating T cells. We show here that T cells in chronic AD skin produce IL-31 and that AD lesions contain increased levels of these IL-31-producing T cells. This suggests that a substantial part of previously reported increased IL-31 mRNA levels in AD skin is T cell derived and that these cells may be involved in the pathogenesis of AD.
Krisztina Szegedi; Andreas E Kremer; Sanja Kezic; Marcel B M Teunissen; Jan D Bos; Rosalie M Luiten; Pieter C Res; Maritza A Middelkamp-Hup
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental dermatology     Volume:  21     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  431-6     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands Coronel Institute for Occupational Health, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
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