| Increased fetal cell microchimerism in high grade breast carcinomas occurring during pregnancy. | |
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MedLine Citation:
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PMID: 19065666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pregnancy results in the transfer of stem cells from the fetus to the maternal circulation. These cells are able to migrate and differentiate within various damaged maternal tissues. We recently showed the presence of fetal-derived cells in human breast carcinomas during pregnancy. In this study, we aimed to reproduce these results in a murine model of "pregnancy-associated" breast carcinoma. We bred virgin MMTV-H-Ras transgenic female mice with male mice transgenic for luciferase under the control of the VEGFR2 promoter. Tumors that developed during or following gestation were analyzed and their nuclear grade classified. Fetal cells were detected by Y chromosome Fluorescence in situ hybridization FISH in 9/9 of breast carcinomas but only in 2 liver controls from the same animals. The number of fetal cells was 20 and 4.9 per million maternal cells in these tissues, respectively (p < 0.05). High grade tumors had significantly more fetal cells (p < 0.05). In vivo imaging of the luciferase signal under control of the VEGFR2 promoter as well as von Willebrand staining did not reveal an endothelial phenotype of fetal cells. Sixty two percent of the fetal cells expressed cytokeratins but were not tumoral. In conclusion, fetal cells-expressing cytokeratin-are always present in murine breast carcinomas associated with gestation. Interestingly, high-grade tumors contain more fetal cells. |
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Authors:
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Gil Dubernard; Michel Oster; Fabrice Chareyre; Martine Antoine; Roman Rouzier; Serge Uzan; Selim Aractingi; Kiarash Khosrotehrani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 124 ISSN: 1097-0215 ISO Abbreviation: Int. J. Cancer Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2008-12-24 Completed Date: 2009-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 1054-9 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiopathologie du développement, UPMC University of Paris, EA, UMR S, Paris, France. gdubernard@yahoo.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chimerism* Female Fetus / cytology* Genes, ras Male Mammary Neoplasms, Experimental / pathology* Mammary Tumor Virus, Mouse Maternal-Fetal Exchange* Mice Pregnancy Pregnancy Complications, Neoplastic / pathology* Salivary Gland Neoplasms / pathology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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