Document Detail

Increased expression of nitric oxide synthase interacting protein (NOSIP) following traumatic spinal cord injury in rats.
MedLine Citation:
PMID:  23135205     Owner:  NLM     Status:  Publisher    
Previous studies indicated that nitric oxide (NO) is involved in secondary damage of spinal cord injury (SCI), which worsens the primary physical injury to the central nervous systems. Recently, nitric oxide synthase interacting protein (NOSIP) has been identified to interact with neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase by inhibiting the NO production. However, its expression and function after a central nervous system injury remains unclear. In this study, we examined the expression and cellular localization of NOSIP in the spinal cord of an adult rat. Western blot analysis indicated that NOSIP protein levels increased at day1 post-injury and peaked at day 14. Double immunofluorescence staining showed that NOSIP was primarily expressed in neurons and glial cells in the intact spinal cord. Interestingly, this study also showed that the expression of NOSIP significantly increased in astrocytes after injury. Furthermore, injury-induced expression of NOSIP was co-expressed with proliferating cell nuclear antigen (PCNA) positive astrocytes after injury. We also showed the NOSIP was co-localized with nNOS in gray matter and white matter after SCI. All these data taken together suggested that NOSIP may play an important roles in astrogliogenesis after a spinal cord injury.
Xiaowei Yu; Yi Zhong; Zhenzhong Zhu; Tianyi Wu; Aiguo Shen; Ye Huang
Related Documents :
24794565 - Expression of each cistron in the gal operon can be regulated by transcription terminat...
19156345 - A molecular biomarker for disruption of crustacean molting: the n-acetyl-beta-glucosami...
17007105 - Glycophenotype of psoriatic skin.
11085505 - Id proteins are dynamically expressed in normal epidermis and dysregulated in squamous ...
1748285 - Nascent pre-mrna transcripts are associated with nuclear regions enriched in splicing f...
20123975 - Rapid-response splicing reporter screens identify differential regulators of constituti...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of molecular histology     Volume:  -     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Orthopaedics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of exercise therapy on knee joint function and synovial fluid cytokine levels in patients wi...
Next Document:  A supramolecularly templated catenane initiator and a controlled ring expansion strategy.