Document Detail


Increased expression of macrophage migration inhibitory factor during progression to hypopharyngeal squamous cell carcinoma.
MedLine Citation:
PMID:  20944103     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: To examine the presence of macrophage migration inhibitory factor (MIF) quantitatively in relation to neoplastic progression of hypopharyngeal squamous cell carcinoma (HSCC). MATERIALS AND METHODS: The presence of MIF was analysed by quantitative immunohistochemistry in sections of 81 HSCCs, and compared to 15 specimens of tumour-free epithelia (TF_E), 29 low-grade dysplasias (Low_D) and 25 high-grade dysplasias (High_D). In parallel, MIF expression was studied using Western blotting on a series of 19 fresh biopsies. RESULTS: A significant increase in MIF staining intensity (mean optical density) was observed in carcinoma samples compared to TF_E (p<10(-6)), Low_D (p=0.0006) or High_D (p=0.0006). Immunohistochemical MIF positivity was significantly higher in HSCCs than in TF_E (p=0.00001) or Low_D (p=0.001). The percentage of MIF-immunopositive cells (labelling index) significantly decreased in parallel with an apparent loss of histological differentiation (p=0.003). CONCLUSION: This study identified the presence of MIF as a parameter that positively correlates with neoplastic progression of HSCC and cell differentiation status.
Authors:
Stéphanie Cludts; Christine Decaestecker; Bryon Johnson; Jérôme Lechien; Xavier Leroy; Nadège Kindt; Herbert Kaltner; Sabine André; Hans-Joachim Gabius; Sven Saussez
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3313-9     Citation Subset:  IM    
Affiliation:
Laboratory of Anatomy and Cellular Biology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Mons, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Carcinoma, Squamous Cell / metabolism,  pathology*
Disease Progression
Female
Humans
Hypopharyngeal Neoplasms / metabolism,  pathology*
Immunohistochemistry
Intramolecular Oxidoreductases / biosynthesis*
Macrophage Migration-Inhibitory Factors / biosynthesis*
Male
Middle Aged
Neoplasm Staging
Tumor Markers, Biological / analysis*
Chemical
Reg. No./Substance:
0/Macrophage Migration-Inhibitory Factors; 0/Tumor Markers, Biological; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.2.1/MIF protein, human

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