Document Detail

Increased expression of interleukin-8 and aminopeptidase N by cell-cell contact: interleukin-8 is resistant to degradation by aminopeptidase N/CD13.
MedLine Citation:
PMID:  11399521     Owner:  NLM     Status:  MEDLINE    
In rheumatic joints, high concentrations of interleukin-8 (IL-8) have been measured in synovial fluid and in pannus tissue. In both locations aminopeptidase N (APN)/CD13, an exopeptidase with reported activity towards IL-8 is also present. The surprising stability of IL-8 in the presence of an alleged IL-8-degrading peptidase prompted us to undertake the present study. Cocultivation of fibroblast-like synoviocytes (SFC) with T cells or with T lymphocytic cell membranes, or of T cells with SFC cell membranes, all resulted in increased IL-8 mRNA expression and IL-8 secretion into the medium, and an increase of APN expression on lymphocytes. IL-8 degradation was monitored by Western blots and HPLC. IL-8(72), as a partially processed form, was used throughout this study since it is abundant in tissues and has increased biological activity in comparison to IL-8(77). Thus its degradation/inactivation is considered of high biological significance. Whereas trypsin as a positive control rapidly degraded IL-8, we did not see any IL-8 degradation, either by a variety of soluble APNs, by leucine aminopeptidase or by APN expressed on the surface of SFC, or on ECV304 cells transfected with an APN expression vector. The much more sensitive HPLC technique resulted in negative results as well.
A Kehlen; I Egbert; K Thiele; K Fischer; D Riemann; J Langner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European cytokine network     Volume:  12     ISSN:  1148-5493     ISO Abbreviation:  Eur. Cytokine Netw.     Publication Date:    2001 Apr-Jun
Date Detail:
Created Date:  2001-06-11     Completed Date:  2001-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100879     Medline TA:  Eur Cytokine Netw     Country:  France    
Other Details:
Languages:  eng     Pagination:  316-24     Citation Subset:  IM    
Martin Luther University, Institute of Medical Immunology, Magdeburger Strasse 2, D-06097 Halle, Germany.
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MeSH Terms
Antigens, CD13 / genetics,  metabolism*
Cell Communication*
Chromatography, High Pressure Liquid
DNA-Binding Proteins / genetics
I-kappa B Proteins*
Interleukin-2 / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / metabolism
Reg. No./Substance:
0/DNA-Binding Proteins; 0/I-kappa B Proteins; 0/Interleukin-2; 0/RNA, Messenger; 139874-52-5/NF-kappaB inhibitor alpha; EC, CD13

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