Document Detail


Increased expression of inducible nitric oxide synthase in circulating monocytes from patients with active inflammatory bowel disease.
MedLine Citation:
PMID:  12059056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) synthesis are increased in epithelial cells and in tissue macrophages of the inflamed mucosa from patients with inflammatory bowel disease (IBD). Since tissue macrophages are derived from circulating monocytes, we studied iNOS expression in circulating monocytes and related this expression to disease activity. In view of the possible role of NO in monocyte function, we also studied iNOS expression in relation to markers of monocyte activation.
METHODS: The expression of iNOS in circulating monocytes from 15 patients with active IBD, 6 patients who went into remission and 18 healthy controls was quantified by flow cytometry and correlated with surface markers (CD63, CD11b, HLA-DR) for monocyte activation. In addition, iNOS expression in circulating monocytes was assessed by Western blotting, immunocytochemistry and measurement of the NO metabolites nitrite and nitrate in plasma.
RESULTS: The expression of iNOS in circulating monocytes and the percentage of iNOS-positive monocytes were increased in patients with active IBD compared to healthy controls (fluorescence index: 1.3 (0.1-6.3) versus 0.8 (0.0-1.8); P < 0.05: percentage of iNOS positive monocytes: 37.3 (1.0-77.0)% versus 5.3 (0.0-43.3)%; P<0.01). The six patients who went into remission all had a marked reduction of iNOS expression. iNOS expression was confirmed by Western blotting and immunocytochemistry. Plasma nitrite and nitrate levels were elevated in three patients with active 1BD. The surface markers for monocyte activation, CD63 and CD11b, were not elevated. HLA-DR expression was decreased on circulating monocytes from patients with active ulcerative colitis.
CONCLUSIONS: iNOS is increased in circulating monocytes from patients with active IBD and this increased expression correlates with disease activity. Considering the decreased HLA-DR expression and absence of monocyte activation markers, NO produced by iNOS may have a function in suppressing systemic monocyte activation.
Authors:
G Dijkstra; A J H Zandvoort; A C Muller Kobold; A de Jager-Krikken; P Heeringa; H van Goor; H M van Dullemen; J W Cohen Tervaert; A van de Loosdrecht; H Moshage; P L M Jansen
Related Documents :
18358626 - Cortical spreading depression induces the expression of inos, hif-1alpha, and ldh-a.
18372436 - Over-expression of gtp-cyclohydrolase 1 feedback regulatory protein attenuates lps and ...
9144076 - Expression of nitric oxide synthase in human peripheral blood mononuclear cells and neu...
10341846 - Expression of inducible nitric oxide synthase in human coronary atherosclerotic plaque.
22143106 - Effects of hypoxic living and training on gene expression in an obese rat model.
7909276 - Platelet-derived growth factor-induced expression of messenger rna for the proliferatin...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  37     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-12     Completed Date:  2002-12-06     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  546-54     Citation Subset:  IM    
Affiliation:
Dept of Internal Medicine, University Hospital, Groningen, The Netherlands. G.Dijkstra@int.azg.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers / analysis
Female
Flow Cytometry
Humans
Immunohistochemistry
Inflammatory Bowel Diseases / enzymology,  immunology*
Male
Middle Aged
Monocytes / metabolism*
Nitric Oxide Synthase / biosynthesis*
Nitric Oxide Synthase Type II
Chemical
Reg. No./Substance:
0/Biological Markers; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Incidence of inflammatory bowel disease in children in southeastern Norway: a prospective population...
Next Document:  The role of mast cells in the development of 2, 4, 6-trinitrobenzene sulfonic acid-induced colitis i...