Document Detail

Increased expression of a high molecular weight matrix component in rat hepatocellular carcinoma.
MedLine Citation:
PMID:  2820909     Owner:  NLM     Status:  MEDLINE    
The urea extract of the glycoproteins from the extracellular matrix of rat liver has been compared with that of Morris hepatoma 7777. A high molecular weight glycoprotein present in Morris hepatoma 7777 was not found in the extract of liver matrix. Under reducing conditions in SDS-gel electrophoresis this component gave two glycoprotein bands with Mr 53 k and 56 k. The indirect immunofluorescence staining with a monospecific antiserum directed against the component showed its abundant presence in Morris hepatoma 7777 as well as in the less malignant Morris hepatoma 9121 in form of extracellular network structures. The antigen also densely filled some cumuli of cells. In contrast the liver tissue showed only very weak staining of the extracellular areas. The overall distribution of the component could be correlated with the distribution of several hydrolases in the tumor matrix, notably beta-D-glucuronidase.
H H Riese; C Hanski; R Gossrau; W Reutter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry     Volume:  87     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1987  
Date Detail:
Created Date:  1987-11-17     Completed Date:  1987-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  IM    
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
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MeSH Terms
Extracellular Matrix / metabolism*
Fluorescent Antibody Technique
Glucuronidase / metabolism
Glycoproteins / metabolism*
Liver Neoplasms, Experimental / metabolism*
Lysosomes / enzymology
Microbial Collagenase / metabolism
Molecular Weight
Reg. No./Substance:
0/Glycoproteins; EC; EC Collagenase

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