| Increased expression of a high molecular weight matrix component in rat hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 2820909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The urea extract of the glycoproteins from the extracellular matrix of rat liver has been compared with that of Morris hepatoma 7777. A high molecular weight glycoprotein present in Morris hepatoma 7777 was not found in the extract of liver matrix. Under reducing conditions in SDS-gel electrophoresis this component gave two glycoprotein bands with Mr 53 k and 56 k. The indirect immunofluorescence staining with a monospecific antiserum directed against the component showed its abundant presence in Morris hepatoma 7777 as well as in the less malignant Morris hepatoma 9121 in form of extracellular network structures. The antigen also densely filled some cumuli of cells. In contrast the liver tissue showed only very weak staining of the extracellular areas. The overall distribution of the component could be correlated with the distribution of several hydrolases in the tumor matrix, notably beta-D-glucuronidase. |
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Authors:
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H H Riese; C Hanski; R Gossrau; W Reutter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry Volume: 87 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1987 |
Date Detail:
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Created Date: 1987-11-17 Completed Date: 1987-11-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 237-42 Citation Subset: IM |
Affiliation:
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Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Extracellular Matrix / metabolism* Fluorescent Antibody Technique Glucuronidase / metabolism Glycoproteins / metabolism* Liver Neoplasms, Experimental / metabolism* Lysosomes / enzymology Microbial Collagenase / metabolism Molecular Weight Rats Solubility |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; EC 3.2.1.31/Glucuronidase; EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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