| Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. | |
| | |
MedLine Citation:
|
PMID: 23362875 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- α (TNF-α), interferon-γ (IFN-γ) or a combination of TNF-α and IFN-γ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis. |
| | |
Authors:
|
Annesofie Faurschou; Jens Pedersen; Mette Gyldenløve; Steen S Poulsen; Jens J Holst; Jacob P Thyssen; Claus Zachariae; Tina Vilsbøll; Lone Skov; Filip K Knop |
Related Documents
:
|
11436205 - Quantitation of tgf-beta1 mrna in porcine mesangial cells by comparative kinetic rt/pcr... 23439585 - Altered folate receptor 2 expression in uraemic patients on haemodialysis: implications... 21300015 - Uvb light regulates expression of antioxidants and inflammatory mediators in human corn... 12113775 - Competitive quantitative measurement of the ampa receptor gene expression at the single... 11561715 - Nuclear mrna binding proteins couple pre-mrna splicing and post-splicing events. 19367675 - Kaempferol and quercetin, essential ingredients in ginkgo biloba extract, inhibit inter... |
Publication Detail:
|
Type: Letter |
Journal Detail:
|
Title: Experimental dermatology Volume: 22 ISSN: 1600-0625 ISO Abbreviation: Exp. Dermatol. Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: Denmark |
Other Details:
|
Languages: eng Pagination: 150-2 Citation Subset: IM |
Copyright Information:
|
© 2013 John Wiley & Sons A/S. |
Affiliation:
|
Diabetes Research Division, Department of Internal Medicine, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic alterations in RAS-regulated pathway in acral lentiginous melanoma.
Next Document: Staphylococcus aureus skin colonization is promoted by barrier disruption and leads to local inflamm...