Document Detail


Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells.
MedLine Citation:
PMID:  16567203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: An increased expression of immediate early genes, such as the c-fos and c-jun, is observed in some organs after ischemia-reperfusion (I/R) injury. These factors have been revealed to potentially induce apoptosis and proliferation of the postischemic cells. The purpose of this study is to analyze the relationship between the expression patterns of such immediate early genes and the cellular responses in the intestinal epithelial cells (IECs) after I/R stress. METHODS: The rat small intestine was reperfused after 30 minutes ischemia. Semiquantitative reverse transcription-polymerase chain reaction was used to quantify c-fos and c-jun messenger RNAs. The proliferation and apoptosis of IECs were detected by immunohistochemistry and the in situ terminal deoxynucleotidyl transferase-mediated dUTP biotin nick-end labeling method, respectively. RESULTS: The messenger RNA levels of the c-fos and c-jun showed characteristic patterns in the IECs after the I/R stress. The proliferation of the cells was initially observed after the I/R stress, followed by apoptosis of the cells. CONCLUSIONS: The sequential expression patterns of these factors are possibly related to the proliferation and apoptosis of the IECs.
Authors:
Yuichi Shima; Tatsuro Tajiri; Tomoaki Taguchi; Sachiyo Suita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  41     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-28     Completed Date:  2006-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  830-6     Citation Subset:  IM    
Affiliation:
Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. yshima@pedsurg.med.kyushu-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Cell Proliferation*
Epithelial Cells / metabolism*
Intestinal Mucosa / cytology*
Intestine, Small / cytology,  metabolism
Male
Proto-Oncogene Proteins c-fos / biosynthesis*
Proto-Oncogene Proteins c-jun / biosynthesis*
Rats
Rats, Inbred Lew
Reperfusion Injury / metabolism*,  pathology*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun

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