Document Detail


Increased expression of adenylyl cyclase 3 in pancreatic islets and central nervous system of diabetic Goto-Kakizaki rats: a possible regulatory role in glucose homeostasis.
MedLine Citation:
PMID:  23018249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenylyl cyclase 3 (AC3) is expressed in pancreatic islets of the Goto-Kakizaki (GK) rat, a spontaneous animal model of type 2 diabetes (T2D), and also exerts genetic effects on the regulation of body weight in man. In addition to pancreatic islets, the central nervous system (CNS) plays an important role in the pathogenesis of T2D and obesity by regulating feeding behavior, body weight and glucose metabolism. In the present study, we have investigated AC3 expression in pancreatic islets, striatum and hypothalamus of GK rats to evaluate its role in the regulation of glucose homeostasis. GK and Wistar rats at the age of 2.5 mo were used. A group of GK rats were implanted with sustained insulin release chips for 15 d. Plasma glucose and serum insulin levels were measured. AC3 gene expression levels in pancreatic islets, striatum and hypothalamus were determined by using real-time RT-PCR. Results indicated that plasma glucose levels in Wistar rats were found to be similar to insulin-treated GK rats, and significantly lower compared with non-treated GK rats. AC3 expression levels in pancreatic islets, striatum and hypothalamus of GK rats were higher compared with Wistar rats, while the levels were intermediate in insulin-treated GK rats. The AC3 expression display patterns between pancreatic islets and striatum-hypothalamus were similar. The present study thus provides the first evidence that AC3 is overexpressed in the regions of striatum and hypothalamus of brain, and similarly in pancreatic islets of GK rats suggesting that AC3 plays a role in regulation of glucose homeostasis via CNS and insulin secretion.
Authors:
Mohammed Seed Ahmed; Abraham Kovoor; Sofia Nordman; Norhashimah Abu Seman; Tianwei Gu; Suad Efendic; Kerstin Brismar; Claes-Göran Östenson; Harvest F Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-01
Journal Detail:
Title:  Islets     Volume:  4     ISSN:  1938-2022     ISO Abbreviation:  Islets     Publication Date:    2012 Sep-Oct
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-06-25     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  101495366     Medline TA:  Islets     Country:  United States    
Other Details:
Languages:  eng     Pagination:  343-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / genetics,  physiology*
Animals
Brain / enzymology*
Diabetes Mellitus, Type 2 / enzymology*
Glucose / metabolism*
Homeostasis*
Insulin / secretion
Islets of Langerhans / enzymology*
Male
RGS Proteins / genetics,  physiology
RNA, Messenger / analysis
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Insulin; 0/RGS Proteins; 0/RNA, Messenger; 0/regulator of g-protein signaling 9; 50-99-7/Glucose; EC 4.6.1.1/Adenylate Cyclase; EC 4.6.1.1/adenylate cyclase 3
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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