| Increased expression of activation-induced cytidine deaminase is associated with anti-CCP and rheumatoid factor in rheumatoid arthritis. | |
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MedLine Citation:
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PMID: 19703021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rheumatoid arthritis (RA) is associated with higher levels of autoantibodies and IL-17. Here, we investigated if ectopic lymphoid follicles and peripheral blood mononuclear cells (PBMCs) from RA patients exhibit increased activation-induced cytidine deaminase (AID), and if increased AID is correlated with serum levels of autoantibodies and IL-17. The results of immunohistochemical staining showed that organized AID(+) germinal centres were observed in six of the 12 RA synovial samples, and AID(+) cells were found almost exclusively in the B-cell areas of these follicles. Aggregated but not organized lymphoid follicles were found in only one OA synovial sample without AID(+) cells. Significantly higher levels of AID mRNA (Aicda) detected by RT-PCR were found in the PBMCs from RA patients than PBMCs from normal controls (P < 0.01). In the PBMCs from RA patients, AID was expressed predominately by the CD10(+)IgM(+)CD20(+) B-cell population and the percentage of these cells that expressed AID was significantly higher than in normal controls (P < 0.01). AID expression in the PBMCs correlated significantly and positively with the serum levels of rheumatoid factor (RF) (P </= 0.0001) and anti-cyclic citrullinated peptide (CCP) (P = 0.0005). Serum levels of IFN-gamma (P = 0.0005) and IL-17 (P = 0.007), but not IL-4, also exhibited positive correlation with the expression of AID. These results suggest that the higher levels of AID expression in B cells of RA patients correlate with, and may be associated with the higher levels of T helper cell cytokines IFN-gamma and IL-17, leading to the development of anti-CCP and RF. |
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Authors:
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X Xu; H-C Hsu; J Chen; W E Grizzle; W W Chatham; C R Stockard; Q Wu; P A Yang; V M Holers; J D Mountz |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 70 ISSN: 1365-3083 ISO Abbreviation: Scand. J. Immunol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-25 Completed Date: 2009-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: England |
Other Details:
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Languages: eng Pagination: 309-16 Citation Subset: IM |
Affiliation:
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Department of Physiology and Physics, University of Alabama at Birmingham, Birmingham, AL 35294, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Arthritis, Rheumatoid / blood, immunology*, pathology Autoantibodies / blood, immunology* B-Lymphocytes / enzymology*, immunology Cytidine Deaminase / biosynthesis*, genetics, immunology Female Germinal Center / enzymology, immunology Humans Interferon-gamma / blood Interleukin-17 / blood Male Middle Aged Peptides, Cyclic / immunology* Rheumatoid Factor / blood, immunology* T-Lymphocytes, Helper-Inducer / immunology |
| Grant Support | |
ID/Acronym/Agency:
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R01 1AI 071110-01A1/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Interleukin-17; 0/Peptides, Cyclic; 0/cyclic citrullinated peptide; 82115-62-6/Interferon-gamma; 9009-79-4/Rheumatoid Factor; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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